使用葫芦脲包封荧光团的超分辨率成像。

Briant Liza, Maillard Jimmy, Fürstenberg Alexandre

Department of Physical Chemistry, University of Geneva, 1211 Genève 4, Switzerland.

Department of Inorganic and Analytical Chemistry, University of Geneva, 1211 Genève 4, Switzerland.

Chem Commun (Camb). 2024 Nov 21;60(94):13943-13946. doi: 10.1039/d4cc05274a.

Red-emitting oxazine fluorophores are shown to bind to cucurbit[7]uril (CB[7]) with high affinity. Their fluorescence quantum yield and lifetime are thereby enhanced owing to shielding of the dyes from water. Using CB[7] as an imaging additive leads to a larger number of photons detected per molecule in super-resolution experiments with the dye ATTO655.

发红光的恶嗪荧光团被证明能以高亲和力与葫芦[7]脲（CB[7]）结合。由于染料与水的隔离，其荧光量子产率和寿命因此得到提高。在使用染料ATTO655的超分辨率实验中，将CB[7]用作成像添加剂会导致每个分子检测到更多的光子。