Yamauchi N, Ito K, Suko M, Ishii A, Miyamoto T
Int Arch Allergy Appl Immunol. 1986;80(1):76-80. doi: 10.1159/000234029.
In order to examine the blocking activity of IgG2 antibodies to guinea pig for IgE antibodies-induced guinea pig asthma, experiments were carried out as follows. Guinea pigs were passively sensitized intravenously with guinea pig serum containing IgE antibodies to ovalbumin (OA). 8 days after sensitization, IgG2 purified from guinea pigs hyperimmunized with OA was intravenously injected. One hour later, the guinea pigs were challenged by inhalation of OA solution. Asthma attacks were not observed in the guinea pigs, whereas the attacks were observed in guinea pigs passively sensitized with the IgE antibodies but injected IgG2 fraction from normal guinea pigs 1 h before inhalation. These observations suggested that IgG antibodies that increased after immunotherapy might block asthma caused by inhalation of allergens in humans.
为了检测IgG2抗体对豚鼠IgE抗体诱导的豚鼠哮喘的阻断活性，进行了如下实验。用含有抗卵清蛋白（OA）IgE抗体的豚鼠血清对豚鼠进行静脉被动致敏。致敏8天后，静脉注射从用OA超免疫的豚鼠中纯化的IgG2。1小时后，通过吸入OA溶液对豚鼠进行激发。在接受IgG2注射的豚鼠中未观察到哮喘发作，而在用IgE抗体被动致敏但在吸入前1小时注射来自正常豚鼠的IgG2组分的豚鼠中观察到了哮喘发作。这些观察结果表明，免疫治疗后增加的IgG抗体可能会阻断人类吸入过敏原引起的哮喘。
