Kim Su-Jeong, Babola Travis A, Lee Kihwan, Matney Chanel J, Spiegel Alina C, Liew Michael H, Schulteis Eva M, Coye Austin E, Proskurin Mikhail, Kang Hyunwook, Kim Julia A, Chevée Maxime, Lee Kiwoong, Kanold Patrick O, Goff Loyal A, Kim Juhyun, Brown Solange P
Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205, USA.
Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205, USA.
bioRxiv. 2024 Nov 5:2024.11.04.621933. doi: 10.1101/2024.11.04.621933.
To understand neocortical function, we must first define its cell types. Recent studies indicate that neurons in the deepest cortical layer play roles in mediating thalamocortical interactions and modulating brain state and are implicated in neuropsychiatric disease. However, understanding the functions of deep layer 6 (L6b) neurons has been hampered by the lack of agreed upon definitions for these cell types. We compared commonly used methods for defining L6b neurons, including molecular, transcriptional and morphological approaches as well as transgenic mouse lines, and identified a core population of L6b neurons. This population does not innervate sensory thalamus, unlike layer 6 corticothalamic neurons (L6CThNs) in more superficial layer 6. Rather, single L6b neurons project ipsilaterally between cortical areas. Although L6b neurons undergo early developmental changes, we found that their intrinsic electrophysiological properties were stable after the first postnatal week. Our results provide a consensus definition for L6b neurons, enabling comparisons across studies.
为了理解新皮层功能，我们必须首先定义其细胞类型。最近的研究表明，最深层皮层中的神经元在介导丘脑皮质相互作用和调节脑状态方面发挥作用，并且与神经精神疾病有关。然而，由于缺乏对这些细胞类型的公认定义，对深层6（L6b）神经元功能的理解受到了阻碍。我们比较了用于定义L6b神经元的常用方法，包括分子、转录和形态学方法以及转基因小鼠品系，并确定了L6b神经元的核心群体。与更浅层的6层皮质丘脑神经元（L6CThNs）不同，这个群体不支配感觉丘脑。相反，单个L6b神经元在皮质区域之间同侧投射。尽管L6b神经元经历早期发育变化，但我们发现它们的内在电生理特性在出生后第一周后是稳定的。我们的结果为L6b神经元提供了一个共识定义，便于跨研究进行比较。
