Guo Bin, Sun Xiaofei, Tao Shan, Tian Tian, Lei Haozhi
Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200233, China.
Anal Chem. 2024 Dec 10;96(49):19430-19438. doi: 10.1021/acs.analchem.4c03914. Epub 2024 Nov 22.
As medical advancements turn most cancers into manageable chronic diseases, new challenges arise in cancer recurrence monitoring. Detecting circulating tumor cells (CTCs) is crucial for monitoring cancer recurrence, but the current methods are cumbersome and costly. This study developed a new CTC detection system combining DNA aptamer recognition, hybridization chain reaction (HCR) technology, and DNA logic devices, enabling the one-step recognition of CTCs by identifying multiple membrane proteins. After catalytically active Au nanoparticles were attached through reduction synthesis in situ onto the DNA hybridization strands of the CTCs surface, a 3,3',5,5'-tetramethylbenzidine (TMB) colorimetric reaction was used to detect CTCs concentration via peroxidase-like catalysis. With this CTCs detection reporting system, we achieved an LOD of 4 cells/mL using an ultraviolet-visible (UV-vis) spectrophotometer. At certain concentrations, CTCs could even be detected visually without the need for an instrument. The development of this CTCs detection reporting system provided a convenient, reliable, and cost-effective detection strategy for widespread CTCs-based cancer recurrence monitoring.
随着医学进步将大多数癌症转变为可控制的慢性病，癌症复发监测出现了新的挑战。检测循环肿瘤细胞（CTC）对于监测癌症复发至关重要，但目前的方法既繁琐又昂贵。本研究开发了一种新的CTC检测系统，该系统结合了DNA适配体识别、杂交链式反应（HCR）技术和DNA逻辑器件，通过识别多种膜蛋白实现对CTC的一步识别。在通过原位还原合成将具有催化活性的金纳米颗粒附着到CTC表面的DNA杂交链上后，利用3,3',5,5'-四甲基联苯胺（TMB）比色反应通过类过氧化物酶催化来检测CTC浓度。使用这种CTC检测报告系统，我们用紫外可见（UV-vis）分光光度计实现了4个细胞/毫升的检测限。在特定浓度下，甚至无需仪器即可肉眼检测到CTC。这种CTC检测报告系统的开发为基于CTC的广泛癌症复发监测提供了一种便捷、可靠且经济高效的检测策略。
