Wu Bian, Fu Minghui, Du Jinghua, Wang Mengjing, Zhang Siyue, Li Sanhe, Chen Junxiao, Zha Wenjun, Li Changyan, Liu Kai, Xu Huashan, Wang Huiying, Shi Shaojie, Wu Yan, Li Peide, You Aiqing, Zhou Lei
Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan, 430070, China.
Hubei Hongshan Laboratory, Wuhan, 430070, China.
Rice (N Y). 2024 Nov 22;17(1):72. doi: 10.1186/s12284-024-00749-1.
Cold stress has a significantly negative effect on the growth, development, and yield of rice. However, the genetic basis for the differences in the cold tolerance of Xian/indica and Geng/japonica rice seedlings is still largely unknown. In this study, an RIL population was generated by crossing of the cold-tolerant japonica variety Nipponbare and the cold-sensitive indica variety WD16343 for BSA-seq analysis, and a major cold tolerance QTL qCTS11 was identified on chromosome 11. This locus was narrowed to the 584 kb region through fine mapping. Sequence alignment and expression analysis identified the cloned gene COLD11 and a novel cold-related gene OsCTS11. According to the reported functional variation of COLD11, Nipponbare (TCG + 3GCG)×2 presented more GCG repeats in the 1st exon than WD16343 (TCG + 3GCG), partially explaining the difference in cold tolerance between the parents. OsCTS11, encoding a stress enhanced protein based on phylogenetic analysis, was strongly induced by cold stress and located in the chloroplast and the nucleus. oscts11-mutant lines generated via CRISPR/Cas9 system improved the cold tolerance of rice seedlings. Our study not only reveals novel genetic loci associated with cold tolerance, but also provides potentially valuable gene resources for the cultivation of cold-tolerant rice.
冷胁迫对水稻的生长、发育和产量具有显著的负面影响。然而，籼稻和粳稻幼苗耐冷性差异的遗传基础仍 largely 未知。在本研究中，通过将耐冷粳稻品种日本晴与冷敏感籼稻品种 WD16343 杂交构建了一个重组自交系群体用于 BSA-seq 分析，并在第 11 号染色体上鉴定出一个主要的耐冷 QTL qCTS11。通过精细定位将该位点缩小到 584 kb 区域。序列比对和表达分析鉴定出克隆基因 COLD11 和一个新的冷相关基因 OsCTS11。根据报道的 COLD11 的功能变异，日本晴（TCG + 3GCG）×2 在第 1 外显子中的 GCG 重复比 WD16343（TCG + 3GCG）更多，部分解释了亲本之间耐冷性的差异。基于系统发育分析，OsCTS11 编码一种胁迫增强蛋白，受冷胁迫强烈诱导，定位于叶绿体和细胞核。通过 CRISPR/Cas9 系统产生的 oscts11 突变系提高了水稻幼苗的耐冷性。我们的研究不仅揭示了与耐冷性相关的新遗传位点，还为耐冷水稻的培育提供了潜在有价值的基因资源。
