卵巢卵泡冲洗作为一种增加牛卵母细胞产量和胚胎生产的方法。
Ovarian follicular flushing as a means of increasing oocyte yield and embryo production in cattle.
机构信息
Paragon Veterinary Group, Dalston, Cumbria CA7 7JF, UK; and School of Biosciences, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UK.
School of Biosciences, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UK.
出版信息
Reprod Fertil Dev. 2024 Nov;36. doi: 10.1071/RD24125.
Context The number of developmentally competent cumulus-oocyte complexes (COCs) retrieved during Ovum Pick-Up (OPU) determines success in both bovine and human assisted reproduction. Follicular flushing for COC retrieval is practicsed widely in humans but not in cattle. Aims To determine the benefits of follicular flushing in cattle and assess the merits of a novel 16G double-lumen needle ('OxIVF') that flushes laterally to the needle shaft. Methods Experiment 1 flushed 655 antral follicles (≥7mm) from 255 abattoir-derived cattle ovaries. In Experiment 2, 12 Holstein heifers underwent two cycles of OPU in a cross-over design comparing both needle types. In Experiment 3, 11 Holstein heifers underwent two cycles of OPU using the OxIVF needle in a cross-over design: flushing (≥7mm follicles) vs a 'Hybrid' approach of flushing (≥7mm follicles) and aspiration (5-7mm follicles); followed by two cycles of standard follicle aspiration (>5mm follicles). Key results In Experiment 1, COC recovery was greater (P =0.034) for the OxIVF vs Standard needle (mean±s.e.; 74.1±2.10% vs 67.0±2.23%); yield of Grade 1 COCs was also greater (20.1±1.97% vs 8.2±1.38%; P P =0.045) for the OxIVF vs Standard needle (89.1±2.98% vs 79.6±3.47%). Day 6 embryo yield was also greater (P =0.017) for the OxIVF vs Standard needle (87.2±4.38% vs 67.6±6.73%). In Experiment 3, recovery of COCs was greater (P =0.033) for 'Flush' vs 'Aspirate' groups (82.1±5.06% vs 66.2±3.48%). However, number of Day 8 blastocysts for the 'Hybrid' vs 'Flush' approach (9.2±1.39vs 6.5±1.05 per cycle) did not reach statistical significance. Conclusions Follicular flushing using the OxIVF needle, embracing the 'Hybrid' approach, has the potential to increase oocyte retrieval and blastocyst number per donor cycle in cattle but requires further validation. Implications Larger scale studies will seek to confirm benefits of follicular flushing using the OxIVF needle in cattle. Future studies should consider applications in both equine and human assisted reproduction.
背景
在卵母细胞抽吸术（OPU）中获得的具有发育能力的卵丘-卵母细胞复合物（COC）数量决定了牛和人类辅助生殖的成功。卵泡冲洗在人类中广泛应用于 COC 回收，但在牛中并未应用。目的：确定在牛中进行卵泡冲洗的益处，并评估新型 16G 双腔针（“OxIVF”）的优点，该针向针轴侧向冲洗。方法：实验 1 从 255 头屠宰场来源的牛卵巢中冲洗了 655 个（≥7mm）的窦卵泡。在实验 2 中，12 头荷斯坦小母牛以交叉设计进行了两次 OPU 周期，比较了两种针型。在实验 3 中，11 头荷斯坦小母牛以交叉设计进行了两次 OPU 周期，使用 OxIVF 针进行冲洗（≥7mm 卵泡）或冲洗（≥7mm 卵泡）和抽吸（5-7mm 卵泡）的“混合”方法；然后进行两次标准卵泡抽吸（>5mm 卵泡）周期。主要结果：在实验 1 中，OxIVF 与标准针相比，COC 回收率更高（P =0.034）（平均值±s.e.；74.1±2.10% vs 67.0±2.23%）；优质 COC 的产量也更高（20.1±1.97% vs 8.2±1.38%；P P =0.045）OxIVF 与标准针相比（89.1±2.98% vs 79.6±3.47%）。OxIVF 与标准针相比，第 6 天胚胎的产量也更高（P =0.017）（87.2±4.38% vs 67.6±6.73%）。在实验 3 中，与“抽吸”组相比，“冲洗”组的 COC 回收率更高（P =0.033）（82.1±5.06% vs 66.2±3.48%）。然而，“混合”方法中的“Hybrid”与“冲洗”组相比，第 8 天囊胚的数量（每个周期 9.2±1.39vs 6.5±1.05）没有达到统计学意义。结论：使用 OxIVF 针进行卵泡冲洗，采用“混合”方法，有可能增加牛的卵母细胞回收和每个供体周期的囊胚数量，但需要进一步验证。意义：更大规模的研究将旨在确认 OxIVF 针在牛中进行卵泡冲洗的益处。未来的研究应考虑在马和人类辅助生殖中的应用。
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