Tomoda A, Shirasawa E, Yoneyama Y
Hemoglobin. 1986;10(1):33-48. doi: 10.3109/03630268609072469.
It was found that 3-hydroxyanthranilic acid and 3-hydroxykynurenine have the capacity of oxidizing and reducing oxy- and met-hemoglobin. However, tryptophan metabolites such as kynurenine and anthranilic acid in which hydrxyl group is not involved did not oxidize and reduce these hemoglobins. The oxidation of oxyhemoglobin with 3-hydroxyanthranilic acid and 3-hydroxykynurenine was much accelerated in the presence of myo-inositol hexakisphosphate or superoxide dismutase, but was much suppressed in the presence of catalase. Deoxyhemoglobin was not oxidized by these compounds. On the other hand, the reduction of methemoglobin with 3-hydroxyanthranilic acid and 3-hydroxykynurenine proceeded both under aerobic and anaerobic conditions, though the rate of reduction was much faster under aerobic conditions than under anaerobic conditions. The reduction of methemoglobin with these compounds was accelerated by myo-inositol hexakisphosphate, but was partially suppressed by superoxide dismutase under aerobic conditions. On the basis of these results, the paradoxical effects of 3-hydroxyanthranilic acid and 3-hydroxykynurenine are discussed in relation to the mechanism of oxidation and reduction of hemoglobin.
研究发现，3-羟基邻氨基苯甲酸和3-羟基犬尿氨酸具有氧化和还原氧合血红蛋白及高铁血红蛋白的能力。然而，色氨酸代谢产物如不涉及羟基的犬尿氨酸和邻氨基苯甲酸则不能氧化和还原这些血红蛋白。在肌醇六磷酸或超氧化物歧化酶存在的情况下，3-羟基邻氨基苯甲酸和3-羟基犬尿氨酸对氧合血红蛋白的氧化作用大大加速，但在过氧化氢酶存在时则受到很大抑制。脱氧血红蛋白不会被这些化合物氧化。另一方面，3-羟基邻氨基苯甲酸和3-羟基犬尿氨酸对高铁血红蛋白的还原作用在有氧和无氧条件下均能进行，不过在有氧条件下的还原速率比在无氧条件下快得多。肌醇六磷酸能加速这些化合物对高铁血红蛋白的还原作用，但在有氧条件下超氧化物歧化酶会部分抑制这种还原作用。基于这些结果，就血红蛋白氧化和还原机制对3-羟基邻氨基苯甲酸和3-羟基犬尿氨酸的矛盾效应进行了讨论。
