超薄层聚丙烯酰胺凝胶等电聚焦法用于血红蛋白变异体的鉴定。
Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants.
作者信息
Budowle B, Eberhardt P
出版信息
Hemoglobin. 1986;10(2):161-72. doi: 10.3109/03630268609046442.
PMID:3957695
Abstract
An ultrathin-layer polyacrylamide gel isoelectric focusing method for typing hemoglobin is described. This method, which uses Pharmalyte pH 6.7-7.7 and an electrode wick distance of 5.0 cm, clearly resolves the A, F, S, C and a number of rare variant allelic products. The technique is ideal for rapid screening of a large number of fresh blood and/or bloodstained samples.
摘要
本文描述了一种用于血红蛋白分型的超薄层聚丙烯酰胺凝胶等电聚焦方法。该方法使用pH值为6.7 - 7.7的Pharmalyte，电极芯距离为5.0厘米，能清晰分辨出A、F、S、C以及一些罕见的变异等位基因产物。该技术非常适合对大量新鲜血液和/或血迹样本进行快速筛查。
Zotero 插件