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整合代谢组学和蛋白质组学揭示了自拟芪术汤抗肝纤维化的作用及机制。

Integrative metabolomics and proteomics reveal the effect and mechanism of Zi Qi decoction on alleviating liver fibrosis.

机构信息

First Clinical Medical College, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing, 210023, Jiangsu, PR China.

School of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, Jiangsu, PR China.

出版信息

Sci Rep. 2024 Nov 22;14(1):28943. doi: 10.1038/s41598-024-80616-7.

DOI:10.1038/s41598-024-80616-7
PMID:39578538
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11584741/
Abstract

Liver fibrosis is a common progressive liver disease that can cause liver dysfunction and lead to serious complications. Zi Qi decoction (ZQ) is a traditional formulation that exerts pharmacological effects on the treatment of liver fibrosis. However, precise intervention mechanisms remain unclear. The aim of this study was to synergistically harness proteomics and metabolomics techniques to elucidate the specific target of ZQ and its potential mechanism of action. A carbon tetrachloride (CCl)-induced liver fibrosis mouse model was established. Subsequently, the protective effect of ZQ on liver fibrosis mice was evaluated according to histopathological examination and biochemical indicators. Quantitative proteomics based on data independent acquisition (DIA) and non-targeted metabolomic analyses revealed the pharmacodynamic mechanism of ZQ. In addition, various cellular and molecular assays were used to detect changes in glycolysis levels in LSECs and mouse liver fibrosis models. The study results showed that ZQ significantly alleviated CCl-induced liver injury and fibrosis in mice. DIA-based quantitative proteomics and non-targeted metabolomics analyses indicated that ZQ treatment downregulated glycolysis-related proteins such as PKM2, PFKP, and HK2, while regulating glycolysis-related metabolites and pathways. In addition, ZQ down-regulated glycolytic activity in mice with liver fibrosis and in LSECs, and inhibited CXCL1 secretion and neutrophil recruitment. ZQ inhibited LSEC glycolysis and mitigated neutrophil infiltration, thereby playing a therapeutic role in liver fibrosis.

摘要

肝纤维化是一种常见的进行性肝病，可导致肝功能障碍，并导致严重的并发症。自拟芪术汤（ZQ）是一种传统的配方，对治疗肝纤维化具有药理作用。然而，确切的干预机制尚不清楚。本研究旨在协同利用蛋白质组学和代谢组学技术，阐明 ZQ 的具体靶点及其潜在的作用机制。建立了四氯化碳（CCl）诱导的肝纤维化小鼠模型。随后，根据组织病理学检查和生化指标评价 ZQ 对肝纤维化小鼠的保护作用。基于数据非依赖性采集（DIA）的定量蛋白质组学和非靶向代谢组学分析揭示了 ZQ 的药效学机制。此外，还使用各种细胞和分子测定法检测 LSECs 和小鼠肝纤维化模型中糖酵解水平的变化。研究结果表明，ZQ 可显著减轻 CCl 诱导的小鼠肝损伤和纤维化。DIA 定量蛋白质组学和非靶向代谢组学分析表明，ZQ 处理下调了 PKM2、PFKP 和 HK2 等与糖酵解相关的蛋白质，同时调节了与糖酵解相关的代谢物和途径。此外，ZQ 降低了肝纤维化小鼠和 LSECs 中的糖酵解活性，抑制了 CXCL1 的分泌和中性粒细胞的募集。ZQ 抑制 LSEC 的糖酵解并减轻中性粒细胞浸润，从而在肝纤维化中发挥治疗作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ba/11584741/068d8c7deeb1/41598_2024_80616_Figo_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ba/11584741/068d8c7deeb1/41598_2024_80616_Figo_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ba/11584741/a7ec1544a500/41598_2024_80616_Figa_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ba/11584741/8556d7ea4c3d/41598_2024_80616_Fige_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ba/11584741/903e09a77b72/41598_2024_80616_Figf_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ba/11584741/1c553be18b8c/41598_2024_80616_Figg_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ba/11584741/068d8c7deeb1/41598_2024_80616_Figo_HTML.jpg

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本文引用的文献

1
Blockade of KLF5/LDH-A feedback loop contributes to Curcumol inhibition of sinusoidal endothelial cell glycolysis and mitigation of liver fibrosis.阻断 KLF5/LDH-A 反馈环路有助于莪术醇抑制窦状内皮细胞糖酵解和减轻肝纤维化。
Phytomedicine. 2023 Jun;114:154759. doi: 10.1016/j.phymed.2023.154759. Epub 2023 Mar 22.
2
Global burden of liver disease: 2023 update.全球肝病负担：2023 年更新。
J Hepatol. 2023 Aug;79(2):516-537. doi: 10.1016/j.jhep.2023.03.017. Epub 2023 Mar 27.
3
Crosstalk between glucose metabolism, lactate production and immune response modulation.
糖代谢、乳酸生成与免疫应答调节的串扰。
Cytokine Growth Factor Rev. 2022 Dec;68:81-92. doi: 10.1016/j.cytogfr.2022.11.001. Epub 2022 Nov 7.
4
Tandem mass tag-based proteomics analysis reveals the multitarget mechanisms of against liver fibrosis.基于串联质量标签的蛋白质组学分析揭示了[药物名称]抗肝纤维化的多靶点机制。 （注：原文中“against liver fibrosis”前缺少具体药物等相关信息，这里补充了[药物名称]使句子完整通顺）
Front Pharmacol. 2022 Oct 13;13:989995. doi: 10.3389/fphar.2022.989995. eCollection 2022.
5
RNA mA methylation regulates glycolysis of cancer cells through modulating ATP5D.RNA mA 甲基化通过调节 ATP5D 来调节癌细胞的糖酵解。
Proc Natl Acad Sci U S A. 2022 Jul 12;119(28):e2119038119. doi: 10.1073/pnas.2119038119. Epub 2022 Jul 8.
6
Immune cell metabolism and metabolic reprogramming.免疫细胞代谢与代谢重编程。
Mol Biol Rep. 2022 Oct;49(10):9783-9795. doi: 10.1007/s11033-022-07474-2. Epub 2022 Jun 13.
7
Mechanotransduction-induced glycolysis epigenetically regulates a CXCL1-dominant angiocrine signaling program in liver sinusoidal endothelial cells in vitro and in vivo.机械转导诱导的糖酵解通过表观遗传调控肝窦内皮细胞中 CXCL1 优势的血管分泌信号通路，在体外和体内研究中。
J Hepatol. 2022 Sep;77(3):723-734. doi: 10.1016/j.jhep.2022.03.029. Epub 2022 Apr 12.
8
FSTL1 promotes liver fibrosis by reprogramming macrophage function through modulating the intracellular function of PKM2.FSTL1 通过调节 PKM2 的细胞内功能重编程巨噬细胞功能促进肝纤维化。
Gut. 2022 Dec;71(12):2539-2550. doi: 10.1136/gutjnl-2021-325150. Epub 2022 Feb 9.
9
Neutrophils in chronic inflammatory diseases.慢性炎症性疾病中的中性粒细胞。
Cell Mol Immunol. 2022 Feb;19(2):177-191. doi: 10.1038/s41423-021-00832-3. Epub 2022 Jan 17.
10
Interferon-γ Impairs Human Coronary Artery Endothelial Glucose Metabolism by Tryptophan Catabolism and Activates Fatty Acid Oxidation.干扰素-γ 通过色氨酸分解代谢损害人冠状动脉内皮细胞的葡萄糖代谢并激活脂肪酸氧化。
Circulation. 2021 Nov 16;144(20):1612-1628. doi: 10.1161/CIRCULATIONAHA.121.053960. Epub 2021 Oct 12.