Zigmond S H, Tranquillo A W
J Biol Chem. 1986 Apr 25;261(12):5283-8.
N-Formylnorleucylleucylphenylalanine (f-Nle-LeuPhe) bound to rabbit peritoneal polymorphonuclear leukocytes at 4 degrees C exists in at least two compartments that can be differentiated by their off rates. The off rate of one compartment is similar to that of the receptor characterized previously, about 0.4 min-1 (Aswanikumar, S., Corcoran, B., Schiffmann, E., Day, A. R., Freer, R. J., Showell, H. J., Becker, E. L., and Pert, C. B. (1977) Biochem. Biophys. Res. Commun. 74, 810-817; Sullivan, S. J., and Zigmond, S. H. (1980) J. Cell Biol. 85, 703-711); the off rate of the second compartment is about 0.005 min-1. Lysis of the cells at 4 degrees C with 1% Triton does not affect the peptide release from either compartment. Accumulation of peptide at 4 degrees C into the fast off-rate compartment is rapid, reaching a plateau in about 5 min, while peptide in the slow off-rate compartment continues to increase for up to 4 h. The rate of accumulation in the slow off-rate compartment is approximately proportional to the amount of peptide bound to the fast off-rate compartment. Cells lysed at 4 degrees C before binding are still able to accumulate peptide into both compartments. Three possible models to explain the data are presented.
N-甲酰基去甲亮氨酰亮氨酰苯丙氨酸（f-Nle-LeuPhe）在4℃下与兔腹膜多形核白细胞结合，至少存在于两个可通过其解离速率区分的区室中。一个区室的解离速率与先前表征的受体相似，约为0.4分钟-1（阿斯瓦尼库马尔，S.，科科伦，B.，希夫曼，E.，戴，A.R.，弗里尔，R.J.，肖韦尔，H.J.，贝克尔，E.L.，和佩特，C.B.（1977年）《生物化学与生物物理研究通讯》74，810 - 817；沙利文，S.J.，和齐格蒙德，S.H.（1980年）《细胞生物学杂志》85，703 - 711）；第二个区室的解离速率约为0.005分钟-1。在4℃下用1% Triton裂解细胞不会影响肽从任何一个区室的释放。在4℃下肽快速积累到解离速率快的区室中很快，约5分钟达到平台期，而解离速率慢的区室中的肽在长达4小时内持续增加。解离速率慢的区室中的积累速率大致与结合到解离速率快的区室中的肽量成比例。在结合前于4℃裂解的细胞仍能够将肽积累到两个区室中。提出了三种可能解释这些数据的模型。
