Cao Liyan, Teo Desmond, Wang Yuyang, Ye Qingqing, Liu Chang, Ding Chen, Li Xiangyu, Chang Mingxin, Han Yuqing, Li Zhuo, Sun Xu, Huang Qingeng, Zhang Cui-Ying, Foo Jee Loon, Wong Adison, Yu Aiqun
State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, No.29 the 13th Street TEDA, Tianjin 300457, PR China.
Food Chemical and Biotechnology Cluster, Singapore Institute of Technology, Singapore 828608, Singapore.
ACS Synth Biol. 2024 Dec 20;13(12):3842-3856. doi: 10.1021/acssynbio.4c00599. Epub 2024 Nov 23.
Benzylisoquinoline alkaloids (BIAs) are a class of natural compounds found in plants of the family, known for their diverse pharmacological activities. However, the extraction yields of BIAs from plants are limited, and the cost of chemical synthesis is prohibitively high. Recent advancements in systems metabolic engineering and genomics have made it feasible to use microbes as bioreactors for BIAs production. This review explores recent progress in enhancing the production and yields of BIAs in two microbial systems: and . It covers various BIAs, including ()-reticuline, morphinane, protoberberine, and aporphine alkaloids. The review provides strategies and technologies for BIAs synthesis, analyzes current challenges in BIAs research, and offers recommendations for future research directions.
苄基异喹啉生物碱（BIAs）是一类存在于该科植物中的天然化合物，以其多样的药理活性而闻名。然而，从植物中提取BIAs的产量有限，且化学合成成本过高。系统代谢工程和基因组学的最新进展使得利用微生物作为BIAs生产的生物反应器成为可能。本综述探讨了在两种微生物系统中提高BIAs产量和产率的最新进展： 和 。它涵盖了各种BIAs，包括（）-网状番荔枝碱、吗啡烷、原小檗碱和阿朴啡生物碱。该综述提供了BIAs合成的策略和技术，分析了BIAs研究中的当前挑战，并为未来的研究方向提供了建议。
