Nervig R M, Sebring R W, Scheevel K F
J Biol Stand. 1986 Jan;14(1):21-4. doi: 10.1016/s0092-1157(86)80005-1.
A rapid method for enumerating viable Leptospira interrogans serovar pomona cells was investigated using a bacterial adenosine triphosphate (ATP) assay. The ATP was assayed by the luciferin-luciferase bioluminescence reaction. Samples of serovar pomona grown in liquid polysorbate 80-bovine albumin (P80-BA) medium for 1-3 days were analysed for ATP content, culture density (nephelometry), direct cell count and most probable number of viable cells (MPNVC) as determined by the dilution tube technique. A linear relationship was found between ATP content and the number of viable cells over the range of 4 X 10(8) to 8 X 10(9) leptospires/ml. Over this range the correlation coefficient for ATP content versus viable cells (0.96) was similar to the coefficient for culture density versus the number of viable cells. The coefficient for direct counts versus the number of viable cells was smaller. The bioluminescence assay of bacterial ATP is a promising method for enumerating viable leptospires in pure culture.
采用细菌三磷酸腺苷（ATP）检测法，研究了一种快速计数问号钩端螺旋体波摩那血清型活细胞的方法。通过荧光素-荧光素酶生物发光反应检测ATP。分析在液体聚山梨酯80 - 牛白蛋白（P80 - BA）培养基中培养1 - 3天的波摩那血清型样本的ATP含量、培养密度（比浊法）、直接细胞计数以及通过稀释管技术测定的活细胞最可能数（MPNVC）。在4×10⁸至8×10⁹钩端螺旋体/毫升的范围内，发现ATP含量与活细胞数量之间存在线性关系。在此范围内，ATP含量与活细胞的相关系数（0.96）与培养密度与活细胞数量的系数相似。直接计数与活细胞数量的系数较小。细菌ATP的生物发光检测是一种在纯培养中计数活钩端螺旋体的有前景的方法。
