Zhang Xuefeng, Zhang He, Yin Mingxin, Gao Siyang, Xu Mingyang, Du Guodong
College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Plant Sci. 2025 Feb;351:112323. doi: 10.1016/j.plantsci.2024.112323. Epub 2024 Nov 22.
The deposition of anthocyanin plays a crucial role in fruit pigmentation and serves as the primary determinant of pear quality. Various factors influence the synthesis of anthocyanins, with salicylic acid playing a significant role among them. However, the mechanism by which salicylic acid affects anthocyanin synthesis remains unclear. Our study identifies the transcription factor PyWRKY40 as a pivotal regulator of SA-mediated anthocyanin synthesis in the nucleus. The negative regulatory function of this factor lies in its ability to suppress anthocyanin synthesis, thereby exerting an influence on fruit coloration. We have confirmed the direct binding of PyWRKY40 to the PyDFR promoter through Y1H and EMSA experiments. The findings elucidate a signaling regulatory module, PyWRKY40-PyDFR, which is responsive to SA and enhances fruit pigmentation by modulating anthocyanin metabolism. This insight offers a viable approach to enhancing fruit coloration and improving the overall quality of pear fruits.
花青素的沉积在果实色素沉着中起着关键作用，是梨果实品质的主要决定因素。多种因素影响花青素的合成，其中水杨酸起着重要作用。然而，水杨酸影响花青素合成的机制尚不清楚。我们的研究确定转录因子PyWRKY40是细胞核中SA介导的花青素合成的关键调节因子。该因子的负调节功能在于其抑制花青素合成的能力，从而对果实着色产生影响。我们通过酵母单杂交（Y1H）和电泳迁移率变动分析（EMSA）实验证实了PyWRKY40与PyDFR启动子的直接结合。这些发现阐明了一个信号调节模块PyWRKY40-PyDFR，它对SA有响应，并通过调节花青素代谢增强果实色素沉着。这一见解为增强果实着色和提高梨果实的整体品质提供了一种可行的方法。
