OsPUKI, a PfkB protein, regulates seed germination in rice by influencing ABA synthesis.

Rice seed germination is a crucial phase in rice growth and development, but its molecular mechanism has not been fully elucidated. In this study, we investigated the function of rice pfkB family gene OsPUKI in seed germination. Compared with WT (ZH11), ospuki mutants showed delayed seed germination and shorter shoot length. QRT-PCR results showed that OsPUKI was highly expressed in leaves and developing seeds of 21-day after pollination, and was highly expressed at the early stage of seed germination. GC-MS analysis demonstrated that content of abscisic acid (ABA) in ospuki-3 was higher than that in WT. QRT-PCR analysis revealed that ospuki mutants had higher transcription levels of ABA synthesis-related genes OsNCED2, OsNCED3, OsNCED4, and OsZEP1. Furthermore, it was shown that ospuki mutants were more sensitive to fluridone (Flu) during seed germination and seedling growth than WT according to exogenous Flu treatment experiments. In short, our findings suggest that OsPUKI may positively regulates rice seed germination by influencing ABA synthesis.