Hwang Ji Yeon, Kim Mi-Yeon, Cho Jae Youl
Department of Integrative Biotechnology, and Biomedical Institute for Convergence at SKKU, Republic of Korea.
School of Systems Biomedical Science, Soongsil University, Seoul, 06978, Republic of Korea.
J Ethnopharmacol. 2025 Jan 13;339:119155. doi: 10.1016/j.jep.2024.119155. Epub 2024 Nov 22.
Traditional herbal medicine books "Shin Rhong Bon Cho Kyung" and "Hyang Yak Jip Sung Bang" mentioned that Bletilla striata (Thunb.) Rchb.f. (Orchidaceae) was often used as a medicinal plant and is used in oriental medicine to treat wounds, inflammatory symptoms, and ulcers in stomach, lung, and skin. However, systematic studies on its value as a promising anti-inflammatory remedy were not fully elucidated yet.
The eventual goal of this paper was to explore anti-inflammatory and anti-gastritis effects of Bletilla striata and its inhibitory mechanism with an ethanol extract of this plant (Bs-EE).
In vitro study includes nitric oxide (NO) inhibitory test by was Griess assay, cell viability check by MTT assay, mRNA level analysis of inflammatory genes by PCR and RT-PCR, and protein level analysis by Western blotting and CESTA. In vivo analysis was done with a mouse gastritis model triggered by HCl/EtOH. Phytochemical finger printing result was observed by GC/MS-MS.
Our in vitro trials showed that Bs-EE dose-dependently reduced NO production in lipopolysaccharide-, Poly(I:C)-, and Pam3CSK-treated RAW264.7 cells without causing cytotoxicity, as shown by an MTT assay. The levels of inflammation-related genes (iNOS, IL-6, IL-1β) showed meaningful reductions in RT-PCR and real-time PCR. The NF-κB activity enhanced in MyD88-overexpressing HEK293T cells was strongly reduced by Bs-EE. Western blotting results indicated that the Bs-EE suppressed the phosphorylation of IκBα, IKKα/β, AKT, p65, p50, Syk, and Src, which produced anti-inflammatory effects. Both Syk and Src were found to be direct targets of Bs-EE. This extract attenuated the inflammatory effect in a murine acute gastritis model induced by HCl/EtOH.
These findings suggest that an ethanol extract of Bletilla striata could be developed as a promising natural anti-inflammatory drug or health functional food with NF-κB pathway inhibitory activity.
传统草药书籍《新增本草纲目》和《乡药集成方》提到，白及（兰科）常被用作药用植物，在东方医学中用于治疗伤口、炎症症状以及胃、肺和皮肤的溃疡。然而，关于其作为一种有前景的抗炎药物的价值的系统研究尚未充分阐明。
本文的最终目标是探索白及的抗炎和抗胃炎作用及其乙醇提取物（Bs-EE）的抑制机制。
体外研究包括通过Griess法进行一氧化氮（NO）抑制试验、通过MTT法进行细胞活力检查、通过PCR和RT-PCR分析炎症基因的mRNA水平以及通过蛋白质印迹和CESTA进行蛋白质水平分析。体内分析使用由HCl/EtOH引发的小鼠胃炎模型。通过GC/MS-MS观察植物化学指纹图谱结果。
我们的体外试验表明，如MTT试验所示，Bs-EE在脂多糖、聚肌苷酸:胞苷酸（Poly(I:C)）和Pam3CSK处理的RAW264.7细胞中剂量依赖性地降低NO产生，且不引起细胞毒性。RT-PCR和实时PCR显示炎症相关基因（诱导型一氧化氮合酶（iNOS）、白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β））水平有显著降低。Bs-EE强烈降低了在过表达髓样分化因子88（MyD88）的人胚肾293T（HEK293T）细胞中增强的核因子κB（NF-κB）活性。蛋白质印迹结果表明，Bs-EE抑制了IκBα、IKKα/β、蛋白激酶B（AKT）、p65、p50、脾酪氨酸激酶（Syk）和原癌基因酪氨酸蛋白激酶（Src）的磷酸化，从而产生抗炎作用。发现Syk和Src均为Bs-EE的直接靶点。该提取物减轻了由HCl/EtOH诱导的小鼠急性胃炎模型中的炎症作用。
这些发现表明，白及乙醇提取物可开发成为一种有前景的具有NF-κB通路抑制活性的天然抗炎药物或健康功能性食品。
