Aladenika Yetunde Victoria, Akinjiyan Moses Orimoloye, Elekofehinti Olusola Olalekan, Adanlawo Isaac Gbadura
Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti, Nigeria; Department of Science Laboratory Technology, Biochemistry Option, Gateway (ICT) Polytechnic, Sapaade, Ogun state, Nigeria.
Bioinformatics and Molecular Biology Unit, Department of Biochemistry, Federal University of Technology Akure, Akure, Ondo State, Nigeria; Medical Biochemistry, School of Basic Medical Sciences, Federal University of Technology, Akure, Ondo State, Nigeria.
J Ethnopharmacol. 2025 Jan 13;339:119116. doi: 10.1016/j.jep.2024.119116. Epub 2024 Nov 22.
Traditional and medicinal plant treatments for diabetes mellitus (DM) include Bambusa vulgaris (Shrad.), but little is known about the mechanism.
This study investigated the antioxidant and hepatoprotective effects of B. vulgaris.
DM was induced by intraperitoneal injection of streptozotocin (60 mg/kg). Thirty (30) male Wistar rats were then divided into six groups: control; diabetic control; metformin (100 mg/kg); 50, 100, and 200 mg/kg of B. vulgaris (BV) treated. Fasting blood glucose and weights of rats were monitored at three-day intervals and sacrifice was done after twenty-one days. The activities of SOD, CAT, and liver marker enzymes were investigated. The expressions of insulin-sensitive (TGR5, GLP-1), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, ICAM), and antioxidant genes (SOD, CAT) were investigated using RT-PCR. Schrödinger suites and Auto-Dock Vina were used for docking B. vulgaris phytocompounds identified from works of literature with TGR-5. The liver's histology was also assessed.
BV increased antioxidant activities and reduced liver marker activities in the serum. BV downregulated the expressions of genes associated with inflammation and upregulated antioxidant and insulin-sensitive genes relative to diabetic control. BV regenerated the liver architectural tissue degenerated by inflammation due to STZ. B. vulgaris phytocompounds like farobin A (-11.493 kcal/mol), orientin (-12.296 kcal/mol), and rutin (-12.581 kcal/mol) have better binding energy with TGR5 than metformin (-1.961 kcal/mol).
The hepatoprotective and ameliorative effect of B. vulgaris in DM could be due to its ability to boost antioxidant status and insulin secretion and reduce inflammation.
传统的糖尿病（DM）药用植物治疗方法包括使用簕竹（Bambusa vulgaris (Shrad.)），但其作用机制尚不清楚。
本研究调查了簕竹的抗氧化和肝脏保护作用。
通过腹腔注射链脲佐菌素（60mg/kg）诱导糖尿病。然后将30只雄性Wistar大鼠分为六组：对照组；糖尿病对照组；二甲双胍（100mg/kg）组；50、100和200mg/kg簕竹（BV）处理组。每隔三天监测大鼠的空腹血糖和体重，并在21天后处死大鼠。研究了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和肝脏标志物酶的活性。使用逆转录聚合酶链反应（RT-PCR）研究胰岛素敏感性（TGR5、胰高血糖素样肽-1（GLP-1））、促炎细胞因子（肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、细胞间黏附分子（ICAM））和抗氧化基因（SOD、CAT）的表达。使用薛定谔软件包和自动对接软件Vina将从文献中鉴定出的簕竹植物化合物与TGR-5进行对接。还评估了肝脏的组织学。
与糖尿病对照组相比，簕竹增加了抗氧化活性，降低了血清中肝脏标志物的活性。簕竹下调了与炎症相关基因的表达，并上调了抗氧化和胰岛素敏感性基因的表达。簕竹使因链脲佐菌素炎症而退化的肝脏结构组织得以再生。簕竹植物化合物如法罗宾A（-11.493千卡/摩尔）、荭草素（-12.296千卡/摩尔）和芦丁（-12.581千卡/摩尔）与TGR5的结合能比二甲双胍（-1.961千卡/摩尔）更好。
簕竹对糖尿病的肝脏保护和改善作用可能归因于其提高抗氧化状态、促进胰岛素分泌和减轻炎症的能力。
