Centro de Investigação em Química da Universidade do Porto/ Instituto de Ciências Moleculares (CIQUP/IMS), Faculdade de Ciências da Universidade do Porto, Departamento de Química e Bioquímica Rua do Campo Alegre 687, 4169-007, Porto, Portugal; REQUIMTE-LAQV, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, Porto, Portugal.
Centro de Investigação em Química da Universidade do Porto/ Instituto de Ciências Moleculares (CIQUP/IMS), Faculdade de Ciências da Universidade do Porto, Departamento de Química e Bioquímica Rua do Campo Alegre 687, 4169-007, Porto, Portugal.
Anal Chim Acta. 2024 Dec 15;1332:343340. doi: 10.1016/j.aca.2024.343340. Epub 2024 Oct 16.
Biosensors, especially those designed for detecting food allergens like Gly m TI in soybean, play a crucial role in safeguarding individuals who suffer from adverse food allergies, extending to both individual well-being and broader public health considerations. Furthermore, their integration into food production and monitoring processes aids in compliance with regulatory standards, reducing the incidence of allergen-related recalls and protecting vulnerable populations. Technological advancements in biosensor development, such as increased portability, real-time monitoring capabilities, and user-friendly interfaces, have expanded their practical applications, making them indispensable in various settings, including manufacturing plants, food service establishments, and even at-home use by consumers. For the first time, a biosensor targeting the Gly m TI allergen based on molecularly imprinted polymer (MIP) technology was developed to detect/quantify soybean in complex food matrices and effectively address the detection challenges of complex and processed foods. The Gly m TI-MIP underwent a thorough validation process using anti-Gly m TI IgG raised as a polyclonal response to the trypsin inhibitor. Gly m TI-MIP was successfully tested across a range of food matrices, including tree nuts (e.g., peanuts, walnuts, and hazelnuts) and legumes (e.g., lentils, beans, and lupine), presenting minimal cross-reactivity with lupine and walnut. The innovative approach provided a linear response in the 1 ag mL - 10 μg mL range, with a LOD<1 ag mL. Applying the Gly m TI-MIP sensor to complex model foods allowed to detect 0.1 mg kg (0.00001 %) of soybean protein isolate in biscuits, ham, and sausages before and after the respective thermal treatments. The innovative biosensor can significantly improve food safety protocols by addressing the complexities of tracing allergens in processed and unprocessed food products. By ensuring rigorous allergen control, these biosensors may support global food trade compliance with international safety standards, boost consumer confidence, and promote transparency in food labeling, ultimately contributing to a safer food supply chain.
生物传感器，特别是那些专门用于检测食物过敏原（如大豆中的 Gly m TI）的生物传感器，在保护那些患有食物过敏的个人方面发挥着至关重要的作用，既关乎个人健康，也关乎更广泛的公共健康考虑。此外，将其整合到食品生产和监测过程中有助于符合监管标准，减少与过敏原相关的召回事件，并保护弱势群体。生物传感器开发方面的技术进步，如提高便携性、实时监测能力和用户友好的界面，扩展了其实际应用，使它们在各种场景中变得不可或缺，包括制造工厂、食品服务场所，甚至消费者在家中使用。首次开发了一种基于分子印迹聚合物（MIP）技术的针对 Gly m TI 过敏原的生物传感器，用于检测/定量复杂食品基质中的大豆，并有效地解决了复杂加工食品的检测挑战。Gly m TI-MIP 经过了使用针对胰蛋白酶抑制剂的多克隆反应产生的抗 Gly m TI IgG 的彻底验证过程。Gly m TI-MIP 在一系列食品基质中进行了成功测试，包括坚果（如花生、核桃和榛子）和豆类（如扁豆、豆类和羽扇豆），与羽扇豆和核桃的交叉反应最小。该创新方法在 1 ag mL - 10 μg mL 范围内提供了线性响应，检出限<1 ag mL。将 Gly m TI-MIP 传感器应用于复杂模型食品中，可在饼干、火腿和香肠进行和不进行各自的热处理前后，检测到 0.1 mg kg（0.00001%）的大豆分离蛋白。这种创新的生物传感器可以通过解决加工和未加工食品产品中追踪过敏原的复杂性，显著改善食品安全协议。通过确保严格的过敏原控制，这些生物传感器可以支持全球食品贸易符合国际安全标准，增强消费者信心，并促进食品标签的透明度，最终有助于建立更安全的食品供应链。
