Kramer Ashley E, Ellwood Kathryn M, Guarino Nicole, Li Cong-Jun, Dutta Aditya
Department of Animal & Food Sciences, University of Delaware, Newark, DE, 19716, USA.
Departments of Animal & Food Sciences and Communication Sciences & Disorders, University of Delaware, Newark, DE, 19716, USA.
Poult Sci. 2025 Jan;104(1):104547. doi: 10.1016/j.psj.2024.104547. Epub 2024 Nov 13.
Understanding the mechanisms of follicular recruitment is essential for improving laying hen and broiler breeder productivity, as it directly influences egg production. Despite advancements in poultry breeding for enhanced egg production, the factors driving successful ovarian follicle maturation remain inadequately understood. This study investigates the genetic drivers mediating the transition of pre-recruitment follicles to the pre-ovulatory phase, a crucial stage before ovulation. Using RNA sequencing and bioinformatics approaches such as a differential gene expression analysis, we compared pre-recruitment follicles with the recently recruited F5 pre-ovulatory follicle to identify key genes and upstream regulators involved in this transition. Further validation through qRT-PCR confirmed these findings. Using Qiagen's Ingenuity Pathway Analysis we identified MYC proto-oncogene (C-Myc) as a pivotal upstream regulator, controlling genes essential for cell proliferation and differentiation. Additionally, TGFβ1 emerged as a key regulator, influencing pathways involving SMAD3, TNF, and TP53. The study highlights the intricate regulatory network involving MYC and other transcription factors such as CTNNB1, crucial for follicular development. These findings provide valuable insights into the molecular mechanisms governing follicular selection and maturation, which are essential for enhancing egg production efficiency. Future research should explore the roles of MYC, CTNNB1, and other driver genes in follicular development to further understand and improve reproductive efficiency in poultry.
了解卵泡募集机制对于提高蛋鸡和肉种鸡的生产性能至关重要，因为它直接影响产蛋量。尽管家禽育种在提高产蛋量方面取得了进展，但驱动卵巢卵泡成功成熟的因素仍未得到充分了解。本研究调查了介导募集前卵泡向排卵前阶段转变的遗传驱动因素，排卵前阶段是排卵前的关键阶段。使用RNA测序和生物信息学方法，如差异基因表达分析，我们将募集前卵泡与最近募集的F5排卵前卵泡进行比较，以确定参与这一转变的关键基因和上游调节因子。通过qRT-PCR进一步验证证实了这些发现。使用Qiagen的 Ingenuity Pathway Analysis，我们确定MYC原癌基因（C-Myc）是一个关键的上游调节因子，控制着细胞增殖和分化所必需的基因。此外，TGFβ1成为一个关键调节因子，影响涉及SMAD3、TNF和TP53的信号通路。该研究突出了涉及MYC和其他转录因子（如CTNNB1）的复杂调控网络，这对卵泡发育至关重要。这些发现为控制卵泡选择和成熟的分子机制提供了有价值的见解，这对提高产蛋效率至关重要。未来的研究应探索MYC、CTNNB1和其他驱动基因在卵泡发育中的作用，以进一步了解和提高家禽的繁殖效率。
