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四川红茶加工过程中关键挥发性化合物与核心功能细菌群落的相关性分析

Correlation analysis between key volatile compounds and core functional bacterial community during Sichuan black tea processing.

作者信息

Liao Si-Yu, Yang Shuang, Li Bin-Lin, Xia Xue, Jia Wen-Bao, Zhao Yi-Qiao, Lin Ling, Bian Jin-Lin, Bouphun Tunyaluk, Xu Wei

机构信息

College of Horticulture, Tea Refining and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna Lampang, Lampang 52000, Thailand.

出版信息

Food Chem X. 2024 Nov 5;24:101969. doi: 10.1016/j.fochx.2024.101969. eCollection 2024 Dec 30.

Abstract

Microorganisms participate in the transformation of non-volatile compounds during black tea processing, while their effects on aroma remains unclear. In the present study, 132 KVCs (key volatile compounds) were identified in Sichuan black tea (SBT), of which, linalool and p-cymene were responsible for the citrus-like and sweet aroma of SBT. During the processing of SBT, -, ---, , , and were the dominant bacterial genera. PCoA (principal components analysis)and Anoism (analysis of similarities) indicated that the bacterial community undergone subtle changes in various processes. Additionally, and core functional bacteria contributing to SBT aroma formation, act mainly by secreting enzymes related to amino acid conversion and glycoside hydrolysis. These enzymes include kynureninase, L-cysteate sulfo-lyase, homoserine dehydrogenase, serine/threonine protein phosphatase 1, and aminotransferase. Overall, these findings provide a deep insight into the potential mechanism of aroma formation under bacterial influence during SBT processing.

摘要

微生物参与红茶加工过程中非挥发性化合物的转化,但其对香气的影响尚不清楚。在本研究中,在四川红茶(SBT)中鉴定出132种关键挥发性化合物(KVCs),其中芳樟醇和对伞花烃赋予SBT柑橘样和甜香。在SBT加工过程中,-、---、、和是优势细菌属。主成分分析(PCoA)和相似性分析(Anoism)表明细菌群落在各个过程中发生了细微变化。此外,和是有助于SBT香气形成的核心功能细菌,主要通过分泌与氨基酸转化和糖苷水解相关的酶起作用。这些酶包括犬尿氨酸酶、L-半胱氨酸磺基裂解酶、高丝氨酸脱氢酶、丝氨酸/苏氨酸蛋白磷酸酶1和转氨酶。总体而言,这些发现深入揭示了SBT加工过程中细菌影响下香气形成的潜在机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4335/11584953/53089056d99f/gr1.jpg

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