Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Institute of Biological and Chemical Systems, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Karlsruhe, Germany.
Anim Biotechnol. 2024 Nov;35(1):2429692. doi: 10.1080/10495398.2024.2429692. Epub 2024 Nov 25.
The () gene plays a pivotal role in ovarian development, ovulation, and reproductive traits. There is a lack of studies on its impact on ovarian traits and reproductive traits in cattle. This study aimed to explore gene polymorphisms associations with reproductive traits and investigate the distribution of gene polymorphisms across diverse bovine breeds worldwide. We identified a novel 17-bp deletion within the gene specifically in Chinese Holstein cows (n = 1033) leading to the observation of two genotypes DD and ID. Subsequent association analysis revealed a significant correlation between the 'ID' genotype at this locus and a larger number of corpus albicans ( = 0.042) in diestrus, as well as a higher number of mature follicles ( = 0.038) in estrus. In addition, we also found that the distribution of this deletion exhibits strong regionality across different cattle breeds globally. These findings indicate that the 17-bp deletion mutation within the gene is significantly associated with an increased corpus luteum diameter and a greater number of mature follicles, suggesting its potential utility as a valuable DNA marker for enhancing cow fertility.
（）基因在卵巢发育、排卵和生殖特征中起着关键作用。目前关于其对牛卵巢特征和生殖特征的影响的研究还很少。本研究旨在探讨基因多态性与生殖特征的关系，并研究该基因多态性在世界不同牛种中的分布情况。我们在荷斯坦奶牛中发现了一个新的 17 个碱基对的缺失，导致了两种基因型 DD 和 ID 的观察。随后的关联分析表明，在这个位点上的“ID”基因型与发情期的黄体数量较多（=0.042）以及发情期的成熟卵泡数量较多（=0.038）之间存在显著相关性。此外，我们还发现，这种缺失的分布在全球不同的牛种中具有很强的地域性。这些发现表明，基因内的 17 个碱基对缺失突变与黄体直径增大和成熟卵泡数量增多显著相关，表明它可能作为一种有价值的 DNA 标记，用于提高奶牛的繁殖力。
