Checchia Ilaria, Andreolli Marco, Lanza Francesco, Santoiemma Giacomo, Mori Nicola, Pasini Massimiliano, Lampis Silvia, Felis Giovanna E
Department of Biotechnology, University of Verona, Verona, Italy.
VUCC-DBT Verona University Culture Collection, Department of Biotechnology, University of Verona, Verona, Italy.
Pest Manag Sci. 2025 Mar;81(3):1615-1623. doi: 10.1002/ps.8564. Epub 2024 Nov 25.
The brown marmorated stink bug Halyomorpha halys has become an invasive insect pest of many crops. A promising control strategy to manage the proliferation of H. halys is based on the suppression of its obligate and vertically transmitted uncultivated symbiotic bacterium Candidatus Pantoea carbekii through surface-sterilization of H. halys eggs. Indeed, the application of antimicrobial formulations on the eggs of H. halys could cause mortality of endosymbiont and consequently of newly emerged nymphs. In this study, a microbial live/dead assay was applied directly on H. halys eggs to evaluate Ca. P. carbekii loss of viability after treatments with seven commercial formulations including fungicides (copper hydroxide, sulphur, sweet orange essential oil) and plant biostimulants (flavonoids and chestnut tannin extract) compared with two disinfectants for civil and industrial use (sodium hypochlorite/hydrated sodium/tetraborate decahydrate and peracetic acid/hydrogen peroxide). Impact of mode of application was also evaluated, as surface treatment of egg masses was performed through spraying and dipping in laboratory conditions. Antimicrobial activity data were finally complemented with observations of egg hatching and vitality of the nymphs.
The optimization of live/dead staining is useful for evaluating Ca. P. carbekii mortality directly on eggs, providing a rapid and reliable culture-independent approach. Sodium hypochlorite, copper, sulphur, tannins and sweet orange essential oil showed an antimicrobial effect against Ca. P. carbekii and a H. halys egg hatching reduction and nymph's vitality.
The antimicrobial and insecticidal effects of these commercial products should be further studied to assess their in-field efficiency as well as the impact of these substances on non-target organisms. The approach followed in this study could be considered a robust pipeline of analyses to evaluate the effectiveness of antimicrobial eco-friendly compounds in symbiotic control of H. halys. © 2024 Society of Chemical Industry.
褐边绿刺蛾已成为多种作物的入侵性害虫。一种有前景的控制褐边绿刺蛾种群增长的策略是通过对其卵进行表面消毒来抑制其专性且垂直传播的未培养共生细菌“卡氏泛菌”。实际上，在褐边绿刺蛾卵上施用抗菌制剂可能会导致内共生菌死亡，进而导致新孵化若虫死亡。在本研究中，将微生物活/死检测直接应用于褐边绿刺蛾卵，以评估在用七种商业制剂（包括杀菌剂（氢氧化铜、硫磺、甜橙精油）和植物生物刺激剂（类黄酮和栗木单宁提取物））处理后“卡氏泛菌”的活力丧失情况，并与两种民用和工业用消毒剂（次氯酸钠/十水合硼砂和过氧乙酸/过氧化氢）进行比较。还评估了施用方式的影响，因为在实验室条件下通过喷雾和浸泡对卵块进行了表面处理。抗菌活性数据最终通过观察卵孵化情况和若虫活力得到补充。
活/死染色的优化对于直接评估卵上“卡氏泛菌”的死亡率很有用，提供了一种快速且可靠的无需培养的方法。次氯酸钠、铜、硫磺、单宁和甜橙精油对“卡氏泛菌”显示出抗菌作用，并降低了褐边绿刺蛾卵的孵化率和若虫活力。
这些商业产品的抗菌和杀虫效果应进一步研究，以评估其田间效率以及这些物质对非靶标生物的影响。本研究采用的方法可被视为评估抗菌环保化合物对褐边绿刺蛾共生控制有效性的强大分析流程。© 2024化学工业协会。
