Dai Jiangqin, Guan Haixing, Zhang Linlin, Jiang Hehe, Su Wen, Wang Jue, Jia Xiaolei, Pang Zheng
Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, China.
Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan, China.
J Med Food. 2025 Jan;28(1):44-57. doi: 10.1089/jmf.2024.k.0153. Epub 2024 Nov 25.
, an opportunistic pathogen, commonly causes hospital-acquired pneumonia. Royal jelly fatty acids (RJFAs), a mixture of various fatty acids extracted from royal jelly, exhibit antibacterial and anti-inflammatory properties in treating many infectious diseases. Nevertheless, the therapeutic mechanisms of RJFAs in treatment of acute pulmonary infection are still unclear. Herein, we initially extracted the fatty acids from royal jelly and characterized their chemical constituents using headspace gas chromatography-mass spectrometry. Furthermore, we examined the antibacterial effect of RJFAs and explored its therapeutic effect and molecular mechanisms in treating acute pulmonary infection . The antibacterial studies revealed that RJFAs significantly inhibited growth. Moreover, the studies showed that the RJFAs effectively mitigated the lung damage and inflammation induced by through impairing neutrophil infiltration, reducing the bacterial load in lung and diminishing the production of proinflammatory cytokines, including tumor necrosis factor (TNF-α), interleukin (IL-1β), IL-6, and macrophage inflammatory protein-2 (MIP-2). In addition, the mice treated with RJFAs exhibited reduced phosphorylation of extracellular signal-regulated kinase (ERK), p38, c-Jun N-terminal kinase (JNK), c-Jun, and nuclear factor-kappa B (NF-κB) p65 in the lung tissues in comparison with that of the mice without drug treatment. These findings demonstrated that RJFAs exhibited significant antibacterial and anti-inflammatory effects in treating the -induced acute pneumonia, and the anti-inflammatory effects were exerted through suppressing the mitogen-activated protein kinase/activator protein-1 (MAPK/AP-1) pathway and NF-κB activation, suggesting a promising therapeutic potential of RJFAs against acute bacterial pneumonia.
[一种机会致病菌，通常会引发医院获得性肺炎。蜂王浆脂肪酸（RJFAs）是从蜂王浆中提取的多种脂肪酸的混合物，在治疗多种传染病方面具有抗菌和抗炎特性。然而，RJFAs治疗急性肺部感染的机制仍不清楚。在此，我们首先从蜂王浆中提取脂肪酸，并使用顶空气相色谱 - 质谱法对其化学成分进行表征。此外，我们研究了RJFAs的抗菌作用，并探讨了其在治疗急性肺部感染中的治疗效果和分子机制。抗菌研究表明，RJFAs显著抑制生长。此外，研究表明RJFAs通过损害中性粒细胞浸润、降低肺部细菌载量以及减少促炎细胞因子的产生，包括肿瘤坏死因子（TNF-α）、白细胞介素（IL-1β）、IL-6和巨噬细胞炎性蛋白-2（MIP-2），有效减轻了[某种物质]诱导的肺损伤和炎症。此外，与未接受药物治疗的小鼠相比，接受RJFAs治疗的小鼠肺组织中细胞外信号调节激酶（ERK）、p38、c-Jun氨基末端激酶（JNK）、c-Jun和核因子-κB（NF-κB）p65的磷酸化水平降低。这些发现表明，RJFAs在治疗[某种物质]诱导的急性肺炎中表现出显著的抗菌和抗炎作用，其抗炎作用是通过抑制丝裂原活化蛋白激酶/活化蛋白-1（MAPK/AP-1）途径和NF-κB激活来实现的，这表明RJFAs在治疗急性细菌性肺炎方面具有广阔的治疗潜力。]
原文中部分关键信息缺失（如“an opportunistic pathogen”前缺少具体指代，“through impairing neutrophil infiltration, reducing the bacterial load in lung and diminishing the production of proinflammatory cytokines, including tumor necrosis factor (TNF-α), interleukin (IL-1β), IL-6, and macrophage inflammatory protein-2 (MIP-2).”中缺少引发这些反应的物质），翻译时已根据上下文进行了合理推测补充，但可能与原文完整准确含义存在一定偏差。完整准确的翻译需补充完整这些关键信息。
