Division of Microbiology and Biotechnology, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, 575018, India.
J Med Microbiol. 2024 Nov;73(11). doi: 10.1099/jmm.0.001931.
is a common pathogen associated with many oral diseases and is often isolated from oral cancer patients. However, limited information is available on its key virulence gene expression in oral cancer cell microenvironment and cancer cell behaviour in co-culture studies. overexpresses virulence genes when co-cultured with oral cancer cells and possibly alters the tumour microenvironment, promoting oral cancer proliferation and survival. To investigate altered virulence gene expression in and oral cancer cell behaviour using co-culture experiments. Cal27 cells were co-cultured with and assessed for their cell proliferation, apoptosis, migration and clonogenicity using standard cell culture assays. The levels of reactive oxygen species (ROS) and inflammatory cytokines, along with proliferative, angiogenic and apoptotic biomarker expressions, were also assessed. adherence to cancer cells was demonstrated by the gentamicin protection assay. Real time-PCR was used to analyse the expression of virulence genes. Co-culture of Cal27 cells with showed significantly higher cell proliferation, migration and clonogenicity compared to the control (<0.01). A significant increase in the levels of ROS and inflammatory cytokines and overexpression of Ki67, vascular endothelial growth factor, extracellular signal-regulated kinase 1/2, phosphoinositide 3 kinase and Akt was observed in the co-culture group. also downregulated and genes while upregulated . The virulence genes , and were overexpressed in co-cultured with Cal27 cells. The results from this study indicate the possible risks of infection in oral cancer. An effective antibiotic strategy against to prevent complications associated with oral diseases, including cancer, is needed.
是一种与许多口腔疾病相关的常见病原体，常从口腔癌患者中分离出来。然而，关于其在口腔癌细胞微环境中的关键毒力基因表达以及在共培养研究中对癌细胞行为的影响，信息有限。在与口腔癌细胞共培养时，会过度表达毒力基因，并可能改变肿瘤微环境，促进口腔癌细胞的增殖和存活。为了研究在共培养实验中 与口腔癌细胞行为改变相关的毒力基因表达。将 Cal27 细胞与 共培养，并使用标准细胞培养测定法评估其细胞增殖、凋亡、迁移和集落形成能力。还评估了活性氧 (ROS) 和炎症细胞因子的水平，以及增殖、血管生成和凋亡生物标志物的表达。通过庆大霉素保护试验证明了 对癌细胞的黏附。实时 PCR 用于分析毒力基因的表达。与对照相比，Cal27 细胞与 共培养显示出显著更高的细胞增殖、迁移和集落形成能力（<0.01）。共培养组中观察到 ROS 和炎症细胞因子水平显著增加，Ki67、血管内皮生长因子、细胞外信号调节激酶 1/2、磷酸肌醇 3 激酶和 Akt 的表达上调。还下调了 和 基因，而上调了 。在与 Cal27 细胞共培养时，毒力基因 、 和 过度表达。这项研究的结果表明 感染口腔癌的潜在风险。需要针对 的有效抗生素策略来预防与口腔疾病相关的并发症，包括癌症。
