Fang Beicheng, Lu Lu, Zhao Minjie, Luo Xiaohu, Jia Fuhuai, Feng Fengqin, Wang Jing
Ningbo Innovation Center, Zhejiang University, Ningbo 315000, China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Food Funct. 2024 Dec 9;15(24):12169-12179. doi: 10.1039/d4fo03481c.
Mulberry () is a traditional Chinese fruit that has beneficial effects due to its numerous biological activities. This study aimed to investigate the anti-hyperuricemic activity and underlying mechanism of laboratory-prepared mulberry water extract in mice with hyperuricemia (HUA). Additionally, the effect of mulberry extract (ME) on the microbiota was investigated. The results demonstrated that ME reduced the levels of HUA-related biochemical indices [uric acid (UA), creatinine (Cr), and blood urea nitrogen (BUN)] and pro-inflammatory factors (TNF-α, IL-6, IL-8, and IL-1β) in the serum of HUA model mice. ME suppressed xanthine oxidase (XOD) and adenosine deaminase (ADA) activity while modulating the expression of the urate transporters ATP-binding cassette transporter G2 (ABCG2) and recombinant urate transporter 1 (URAT1) in the kidney. Furthermore, high-dose ME modulated the microbiota, including , , and . Overall, these results demonstrate the efficacy of ME in alleviating HUA by inhibiting XOD and ADA activity, as well as modulating transport proteins to decrease urate synthesis. Additionally, ME regulates the microbiota associated with host UA metabolism. These findings confirm the UA-lowering effects of ME, highlighting its potential as a therapeutic agent for HUA.
桑椹是一种具有多种生物活性而具有有益功效的传统中国水果。本研究旨在探讨实验室制备的桑椹水提取物对高尿酸血症（HUA）小鼠的抗高尿酸血症活性及其潜在机制。此外，还研究了桑椹提取物（ME）对微生物群的影响。结果表明，ME降低了HUA模型小鼠血清中与HUA相关的生化指标[尿酸（UA）、肌酐（Cr）和血尿素氮（BUN）]以及促炎因子（TNF-α、IL-6、IL-8和IL-1β）的水平。ME抑制了黄嘌呤氧化酶（XOD）和腺苷脱氨酶（ADA）的活性，同时调节了肾脏中尿酸转运蛋白ATP结合盒转运体G2（ABCG2）和重组尿酸转运体1（URAT1）的表达。此外，高剂量ME调节了微生物群，包括[此处原文缺失具体微生物名称]、[此处原文缺失具体微生物名称]、[此处原文缺失具体微生物名称]和[此处原文缺失具体微生物名称]。总体而言，这些结果证明了ME通过抑制XOD和ADA活性以及调节转运蛋白以减少尿酸合成来缓解HUA的功效。此外，ME调节与宿主尿酸代谢相关的微生物群。这些发现证实了ME的降尿酸作用，突出了其作为HUA治疗剂的潜力。
