糖鞘脂的短床连续展开薄层色谱法。

Short bed-continuous development thin-layer chromatography of glycosphingolipids.

作者信息

Young W W, Borgman C A

出版信息

J Lipid Res. 1986 Jan;27(1):120-4.

Abstract

The technique of short bed--continuous development chromatography has been utilized to increase the separation of glycosphingolipids on high performance thin-layer chromatography plates. The theoretical goal of increasing separation of bands by decreasing solvent strength was achieved within a practical time span as a result of the high solvent velocities in the short bed tank. Examples are given for increased separation of short and long chain neutral glycolipids, acetylated neutral glycolipids, and gangliosides.

摘要

短床连续展开色谱技术已被用于提高糖鞘脂在高效薄层色谱板上的分离效果。由于短床槽中溶剂流速较高，在实际时间范围内实现了通过降低溶剂强度来增加条带分离度这一理论目标。文中给出了短链和长链中性糖脂、乙酰化中性糖脂以及神经节苷脂分离度提高的示例。