Department of Emergency Medicine, Emergency General Hospital, Beijing, China.
Department of Urology Surgery, Emergency General Hospital, Beijing, China.
Iran J Allergy Asthma Immunol. 2024 Oct 6;23(5):550-562. doi: 10.18502/ijaai.v23i5.16750.
Emodin, derived from Rheum officinale and aloe, is known for its diverse benefits such as anti-inflammatory, antioxidant, and antibacterial properties. Currently, the impact of emodin on urosepsis is unclear. This study aims to investigate the mechanism of action of emodin in urosepsis. Peripheral blood mononuclear cells (PBMCs) were purchased from Cloud-Clone Animal Inc. and treated with emodin. Cell viability and the lactate dehydrogenase (LDH) level were then assessed. In a separate experiment a urosepsis model was established in Sprague Dawley rats which were subsequently treated with emodin. The levels of oxidative stress-related factors, serum complements and inflammatory factors were measured using commercial kits. Blood urea nitrogen and serum creatinine levels were determined using a fully automatic biochemical analyzer. The levels of pro-inflammatory proteins and AMP-activated protein kinase (AMPK)/Sirtuin 1 (SIRT1) pathway-related proteins were evaluated via Western blot. PBMCs were unaffected by emodin concentrations below 60 μg/mL, and minimal LDH levels were detected in the cells. Emodin attenuated the effects of Escherichia coli and diminished the production of serum complements, oxidative stress-related proteins, and inflammatory factors in PBMCs. Notably, the effects of emodin were lessened by an AMPK pathway inhibitor. Additionally, emodin alleviated oxidative stress, complement system activation, inflammation, and kidney injury in urosepsis rats through the AMPK/SIRT1 signaling pathway. Emodin improved kidney damage in urosepsis rats by activating the AMPK/SIRT1 signaling pathway, which reduced oxidative stress, inflammation, and complement system activation.
大黄素来源于大黄和芦荟，具有抗炎、抗氧化和抗菌等多种功效。目前，大黄素对脓毒症的影响尚不清楚。本研究旨在探讨大黄素在脓毒症中的作用机制。外周血单个核细胞（PBMCs）购自 Cloud-Clone Animal Inc.，并使用大黄素进行处理。然后评估细胞活力和乳酸脱氢酶（LDH）水平。在另一个实验中，建立了脓毒症大鼠模型，并随后用大黄素进行治疗。使用商业试剂盒测量氧化应激相关因素、血清补体和炎症因子的水平。使用全自动生化分析仪测定血尿素氮和血清肌酐水平。通过 Western blot 评估促炎蛋白和 AMP 激活的蛋白激酶（AMPK）/沉默调节蛋白 1（SIRT1）通路相关蛋白的水平。PBMCs 不受大黄素浓度低于 60 μg/mL 的影响，且细胞中的 LDH 水平很低。大黄素减弱了大肠杆菌的作用，并减少了 PBMCs 中血清补体、氧化应激相关蛋白和炎症因子的产生。值得注意的是，AMPK 通路抑制剂减轻了大黄素的作用。此外，大黄素通过 AMPK/SIRT1 信号通路减轻脓毒症大鼠的氧化应激、补体系统激活、炎症和肾损伤。大黄素通过激活 AMPK/SIRT1 信号通路改善脓毒症大鼠的肾损伤，从而减轻氧化应激、炎症和补体系统激活。
