School of Life Sciences and Medicine, Shandong University of Technology, Zibo, China.
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China.
Sci Data. 2024 Nov 25;11(1):1279. doi: 10.1038/s41597-024-04073-7.
Nomascus leucogenys is a critically endangered species of small apes. Here, we sequenced and assembled the male genome of N. leucogenys, using PacBio and Hi-C datasets, with a particular focus on its Y-chromosome. The resulting high-quality haplotype-phased assemblies are at chromosome-scale, with scaffold/contig N50 values of 124.2/102.2 Mb for Haplotype 1 and 121.2/85.67 Mb for Haplotype 2. The assembled Y-chromosome spans 16.06 Mb. BUSCO assessment indicated completeness scores exceeding 95%. We predicted 18,925 protein-coding genes (23,783 mRNAs), including 58 genes on the Y-chromosome. Approximately 50% of the genome comprises repetitive elements. These comprehensive genome datasets will serve as a valuable resource for future studies on the genetics and protection of gibbons and improve our understanding on the evolution of Y-chromosome-related genes in primates.
白头叶猴是极度濒危的小型猿类。在这里，我们使用 PacBio 和 Hi-C 数据集对雄性白头叶猴的基因组进行了测序和组装，特别关注其 Y 染色体。产生的高质量单倍型相位组装达到染色体规模，单倍型 1 的支架/拼接体 N50 值为 124.2/102.2Mb，单倍型 2 的支架/拼接体 N50 值为 121.2/85.67Mb。组装的 Y 染色体跨度为 16.06Mb。BUSCO 评估表明完整性得分超过 95%。我们预测了 18925 个编码蛋白的基因（23783 个 mRNA），其中包括 Y 染色体上的 58 个基因。大约 50%的基因组由重复元件组成。这些全面的基因组数据集将成为未来研究长臂猿遗传学和保护的宝贵资源，并提高我们对灵长类动物 Y 染色体相关基因进化的认识。
