Hashmi R S, Siddiqui A M, Kachole M S, Pawar S S
J Nutr. 1986 Apr;116(4):682-8. doi: 10.1093/jn/116.4.682.
Wistar strain adult male and female rats were given 25, 50 and 75% less food than an ad libitum-fed group of rats for 45 d and the effects of food restriction on hepatic drug metabolizing enzymes, microsomal electron transport components, NADPH-dependent lipid peroxidation and glutathione-S-transferase activities were studied. Compared to ad libitum-fed controls, the cytochrome P-450 levels were higher in food restricted male rats, while they were lower in food restricted females. The activities of NADPH cytochrome c reductase were lower in food restricted females than in ad libitum-fed controls. The activities of drug metabolizing enzymes, aminopyrine N-demethylase and acetanilide hydroxylase were higher in food restricted males, whereas in food restricted females these activities were lower than in respective groups fed ad libitum. Microsomal, NADPH-dependent lipid peroxidation was higher in 25 and 50% food restricted females while in 50 and 75% food restricted males it was lower than in ad libitum controls of the same sex. The cytosolic glutathione-S-transferase activities were lower in food restricted rats of both the sexes than in the same sexed controls. Another group of male and female rats were given 75% less food than the ad libitum-fed rats and refed for 3 d prior to killing. Here also, the effects of restriction were different between sexes. It is concluded that hepatic microsomal mixed-function oxidase system (MFOS) is altered due to feed restriction and food restriction followed by refeeding, in a sex-related manner.
将Wistar品系成年雄性和雌性大鼠的食物摄入量比随意进食组减少25%、50%和75%，持续45天，研究食物限制对肝脏药物代谢酶、微粒体电子传递成分、NADPH依赖性脂质过氧化和谷胱甘肽-S-转移酶活性的影响。与随意进食的对照组相比，食物限制的雄性大鼠细胞色素P-450水平较高，而食物限制的雌性大鼠细胞色素P-450水平较低。食物限制的雌性大鼠中NADPH细胞色素c还原酶的活性低于随意进食的对照组。食物限制的雄性大鼠中药物代谢酶氨基比林N-脱甲基酶和乙酰苯胺羟化酶的活性较高，而在食物限制的雌性大鼠中，这些活性低于各自随意进食组。微粒体NADPH依赖性脂质过氧化在食物摄入量减少25%和50%的雌性大鼠中较高，而在食物摄入量减少50%和75%的雄性大鼠中低于同性别随意进食的对照组。两性食物限制大鼠的胞质谷胱甘肽-S-转移酶活性均低于同性别对照组。另一组雄性和雌性大鼠的食物摄入量比随意进食的大鼠减少75%，在处死前再喂养3天。同样，限制的影响在两性之间也有所不同。得出的结论是，由于食物限制以及食物限制后再喂养，肝脏微粒体混合功能氧化酶系统（MFOS）以与性别相关的方式发生改变。
