Kawamura Yusuke, Akuta Norio, Yamamoto Shigeki, Eriksson Yasuka, Hosaka Tetsuya, Saitoh Satoshi, Sezaki Hitomi, Suzuki Fumitaka, Ikeda Kenji, Kumada Hiromitsu
Hepatology, Toranomon Hospital, Tokyo, JPN.
Okinaka Memorial Institute for Medical Research, Toranomon Hospital, Tokyo, JPN.
Cureus. 2024 Oct 25;16(10):e72352. doi: 10.7759/cureus.72352. eCollection 2024 Oct.
Background and objective Drug-eluting beads (DEB) have been highly useful in the current treatment strategies for multiple and large hepatocellular carcinomas (HCC) with or without systemic therapy. Recently, smaller beads have become available in Japan. In this study, we aimed to evaluate the most convenient and effective way to reduce the loading time of epirubicin into the drug-eluting beads M1 (DC Bead M1; 70-150 µm). Methods To reduce the loading time of epirubicin into DC Bead M1, we used a method that involved mixing the drug and then agitating the solution using vortexing: Group A: agitated for 15 sec; Group B: agitated for 20 sec; Group C: agitated for 30 sec; and Group D: left at room temperature as the control group. After the loading of epirubicin by each method, the supernatant concentration of epirubicin was assayed at 5, 10, 15, 20, 30, 60, and 120 min. Results Epirubicin loading rates for DC Bead M1 at five min were 99.7% in Group A, 98.7% in Group B, 99.6% in Group C, and 99.5% in Group D. The four groups reached an equilibrium between five and 120 min. Surprisingly, Group D (left at room temperature for five min) showed the same level of epirubicin loading rate compared to that of Groups A, B, and C at five min (=0.566). Morphological analysis showed that there were no significant morphological changes for each agitated time up to 30 sec compared with that observed for the beads left at room temperature. Conclusions The most convenient and effective way for reducing the loading time of epirubicin into DC Bead M1 was observed in Group D (left at room temperature for five min).
背景与目的 药物洗脱微球（DEB）在目前伴有或不伴有全身治疗的多发性和大型肝细胞癌（HCC）治疗策略中具有很高的应用价值。最近，更小的微球已在日本上市。在本研究中，我们旨在评估将表柔比星载入药物洗脱微球M1（DC Bead M1；70 - 150 µm）的最便捷有效的方法。方法 为减少表柔比星载入DC Bead M1的时间，我们采用了一种先混合药物然后通过涡旋搅拌溶液的方法：A组：搅拌15秒；B组：搅拌20秒；C组：搅拌30秒；D组：室温放置作为对照组。通过每种方法载入表柔比星后，在5、10、15、20、30、60和120分钟时测定表柔比星的上清液浓度。结果 5分钟时，DC Bead M1的表柔比星载入率在A组为99.7%，B组为98.7%，C组为99.6%，D组为99.5%。四组在5至120分钟之间达到平衡。令人惊讶的是，D组（室温放置5分钟）在5分钟时的表柔比星载入率与A、B、C组相同（=0.566）。形态学分析表明，与室温放置的微球相比，搅拌时间长达30秒时，各搅拌时间下均未观察到明显的形态学变化。结论 在D组（室温放置5分钟）中观察到了将表柔比星载入DC Bead M1的最便捷有效的方法。
