Telemo E, Weström B, Dahl G, Karlsson B
J Pediatr Gastroenterol Nutr. 1986 Mar-Apr;5(2):305-9.
Lactating rats were given a test solution containing various marker proteins via oral or intravenous routes. Using immunoprecipitation methods for the detection of ovalbumin (OvA), bovine serum albumin (BSA), and bovine gammaglobulins (BIgG) and radioimmunoassay for the analysis of beta-lactoglobulin (beta-LG), these proteins could be found in the blood serum and the milk of the rat 6 h after administration. A specific distribution pattern between the serum and the milk was observed for each protein. The results show that orally fed dietary proteins are able to cross the gut of the lactating rat and can be found in the serum and the milk. It was also observed that oral feeding of the proteins resulted in relatively greater concentrations in the milk than did intravenous administration. This very early presentation to the young of antigenic macromolecules derived from the mother's diet might be of importance for the development of a proper immunological response to common food antigens.
给哺乳期大鼠经口或静脉途径给予含有各种标记蛋白的测试溶液。使用免疫沉淀法检测卵清蛋白（OvA）、牛血清白蛋白（BSA）和牛γ球蛋白（BIgG），并采用放射免疫分析法分析β-乳球蛋白（β-LG），给药6小时后在大鼠血清和乳汁中可发现这些蛋白。每种蛋白在血清和乳汁之间均观察到特定的分布模式。结果表明，经口摄入的膳食蛋白能够穿过哺乳期大鼠的肠道，并可在血清和乳汁中被发现。还观察到，与静脉给药相比，经口摄入蛋白后乳汁中的浓度相对更高。母体饮食中抗原性大分子如此早期地传递给幼崽，可能对于针对常见食物抗原产生适当免疫反应的发育具有重要意义。
