Matsushita Y, Tsukiori M, Suzuki T, Moriguchi I
J Pharm Sci. 1986 Feb;75(2):193-4. doi: 10.1002/jps.2600750220.
Changes in the fluorescence spectra and quantum yield of 1-pyrenebutyric acid (1) induced by human, bovine, rabbit, and dog serum albumins and healthy adult serum, liver-disease patient serum, and renal-disease patient serum were compared at pH 7.4. Human serum albumin, healthy adult serum, and liver-disease patient serum caused marked quenching of the fluorescence of 1-pyrenebutyric acid. On the contrary, bovine, rabbit, and dog serum albumins increased the fluorescence intensity. Renal-disease patient serum also enhanced the intensity but caused quenching of the fluorescence after treatment with charcoal. Thus, 1-pyrenebutyric acid clearly shows interspecies differences in the environment or the structure of the binding site on serum albumin between humans and other animals. Furthermore, it is suggested that some substance which influences the character of the binding site for 1-pyrenebutyric acid exists in the serum of renal-disease patients.
在pH 7.4条件下，比较了人、牛、兔和狗血清白蛋白以及健康成人血清、肝病患者血清和肾病患者血清对1-芘丁酸（1）荧光光谱和量子产率的影响。人血清白蛋白、健康成人血清和肝病患者血清可显著淬灭1-芘丁酸的荧光。相反，牛、兔和狗血清白蛋白则增强了荧光强度。肾病患者血清也增强了荧光强度，但经活性炭处理后导致荧光淬灭。因此，1-芘丁酸清楚地显示了人与其他动物血清白蛋白结合位点的环境或结构存在种间差异。此外，提示肾病患者血清中存在某种影响1-芘丁酸结合位点性质的物质。
