Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in Zebrafish.

Carnitine palmitoyltransferase 1b (Cpt1b) is a crucial rate-limiting enzyme in fatty acid metabolism, but its role and mechanism in early cardiac development remains unclear. Here, we show that regulates cardiomyocyte proliferation during zebrafish development. Knocking out entire coding sequences leads to impaired cardiomyocyte proliferation, while cardiomyocyte-specific overexpression of promotes cardiomyocyte proliferation. RNA sequencing analysis and pharmacological studies identified glutamine synthetase as a key downstream effector of in regulating cardiomyocyte proliferation. Our study elucidates a novel mechanism whereby promotes zebrafish cardiomyocyte proliferation through glutamine synthetase, which provides new perspectives on the significance of fatty acid metabolism in heart development and the interplay between fatty acid and amino acid metabolic pathways.