Cheng Xiaohan, Ju Jingyi, Huang Wenping, Duan Zongyi, Han Yanchao
Institute for Cardiovascular Science and Department of Cardiovascular Surgery of the First Affiliated Hospital of Soochow University, Suzhou Medical College, Soochow University, Suzhou 215000, China.
J Cardiovasc Dev Dis. 2024 Nov 1;11(11):344. doi: 10.3390/jcdd11110344.
Carnitine palmitoyltransferase 1b (Cpt1b) is a crucial rate-limiting enzyme in fatty acid metabolism, but its role and mechanism in early cardiac development remains unclear. Here, we show that regulates cardiomyocyte proliferation during zebrafish development. Knocking out entire coding sequences leads to impaired cardiomyocyte proliferation, while cardiomyocyte-specific overexpression of promotes cardiomyocyte proliferation. RNA sequencing analysis and pharmacological studies identified glutamine synthetase as a key downstream effector of in regulating cardiomyocyte proliferation. Our study elucidates a novel mechanism whereby promotes zebrafish cardiomyocyte proliferation through glutamine synthetase, which provides new perspectives on the significance of fatty acid metabolism in heart development and the interplay between fatty acid and amino acid metabolic pathways.
肉碱棕榈酰转移酶1b（Cpt1b）是脂肪酸代谢中的一种关键限速酶，但其在心脏早期发育中的作用和机制仍不清楚。在此，我们表明，[该酶名称未给出，原文有误]在斑马鱼发育过程中调节心肌细胞增殖。敲除整个[该酶名称未给出，原文有误]编码序列会导致心肌细胞增殖受损，而心肌细胞特异性过表达[该酶名称未给出，原文有误]则会促进心肌细胞增殖。RNA测序分析和药理学研究确定谷氨酰胺合成酶是[该酶名称未给出，原文有误]调节心肌细胞增殖的关键下游效应物。我们的研究阐明了一种新机制，即[该酶名称未给出，原文有误]通过谷氨酰胺合成酶促进斑马鱼心肌细胞增殖，这为脂肪酸代谢在心脏发育中的意义以及脂肪酸和氨基酸代谢途径之间的相互作用提供了新的视角。
