Ye Minsook, Jang Daehyuk, Lee Sun-Young, Kim Kyu-Ri, Rhie Sung Ja, Oh Jin Kyung, Shim Insop
Department of Physiology, College of Medicine, Kyung Hee University, Seoul 02435, Republic of Korea.
Department of Beauty and Health, Halla University, Wonju-si 26404, Republic of Korea.
Curr Issues Mol Biol. 2024 Oct 22;46(11):11772-11782. doi: 10.3390/cimb46110699.
Alzheimer's disease (AD) is a representative neurodegenerative disease characterized by the structural and functional degeneration of neurons. The present study investigated the neuroprotective effect of (ID) extract on trimethyltin (TMT)-induced memory deficit in the rat. Cognitive improving effect and neuronal activity of ID were assessed by using Morris water maze (MWM) test and choline acetyltransferase (ChAT), cAMP-response element-binding protein (CREB) immunohistochemistry. Seven days after TMT injection (8.0 mg/kg, i.p.), each group of rats was administered saline, water extract of ID (WID, 400 or 800 mg/kg, p.o.), ethanol extract of ID (EID, 400 or 800 mg/kg, p.o.), or caffeic acid (CAF, 30 mg/kg, i.p.) daily for fourteen days. : Treatment with EID and CAF produced a significant improvement in escape latency time of the acquisition, and retention time in the target area of the MWM task. Additionally, administration of EID or CAF markedly alleviated TMT-induced loss of ChAT- and CREB-immunoreactive cells in the hippocampus. The results demonstrated that EID has a protective effect against TMT-induced memory deficit, partly through increasing the CREB and cholinergic signaling pathway in the hippocampus. These results suggest that ethanol extracts of ID might be useful for improving cognitive functions in neurodegenerative diseases such as Alzheimer's disease.
阿尔茨海默病（AD）是一种以神经元结构和功能退化为特征的代表性神经退行性疾病。本研究调查了[ID]提取物对三甲基锡（TMT）诱导的大鼠记忆缺陷的神经保护作用。通过莫里斯水迷宫（MWM）试验以及胆碱乙酰转移酶（ChAT）、环磷酸腺苷反应元件结合蛋白（CREB）免疫组织化学来评估[ID]的认知改善作用和神经元活性。在腹腔注射TMT（8.0mg/kg）7天后，每组大鼠每天分别给予生理盐水、[ID]水提取物（WID，400或800mg/kg，口服）、[ID]乙醇提取物（EID，400或800mg/kg，口服）或咖啡酸（CAF，30mg/kg，腹腔注射）处理，持续14天。结果显示：用EID和CAF处理可显著改善获得阶段的逃避潜伏期以及MWM任务中目标区域的停留时间。此外，给予EID或CAF可明显减轻TMT诱导的海马中ChAT和CREB免疫反应性细胞的损失。结果表明，EID对TMT诱导的记忆缺陷具有保护作用，部分是通过增加海马中的CREB和胆碱能信号通路实现的。这些结果提示，[ID]乙醇提取物可能有助于改善阿尔茨海默病等神经退行性疾病中的认知功能。
