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热敏免疫脂质体包裹的DCM根提取物在前列腺癌细胞靶向给药中的有效应用

Effective Use of DCM Root Extract Encapsulated by Thermosensitive Immunoliposomes for Targeted Drug Delivery in Prostate Cancer Cells.

作者信息

Riet Keamogetswe, Adegoke Ayodeji, Mashele Samson, Sekhoacha Mamello

机构信息

Department of Health Sciences, Central University of Technology, Bloemfontein 9300, South Africa.

Department of Pharmacology, University of the Free State, Bloemfontein 9300, South Africa.

出版信息

Curr Issues Mol Biol. 2024 Oct 27;46(11):12037-12060. doi: 10.3390/cimb46110714.

DOI:10.3390/cimb46110714
PMID:39590308
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11593239/
Abstract

The delivery of anticancer drugs using nanotechnology is a promising approach aimed at improving the therapeutic efficacy and reducing the toxicity of chemotherapeutic agents. Liposomes were prepared using HSPC: DSPE-PEG-2000: DSPE-PEG2000-maleimide in the ratio of 4:1:0.2 and conjugated with a PSA antibody. extract (EME), doxorubicin (Dox), and docetaxel (Doc) encapsulated in temperature-sensitive immunoliposomes were investigated for their activities against the prostate cancer LNCap and DU145 cell lines. Organic extracts of EME leaves, roots, and stems were screened against both cell lines, inhibiting more than 50% of cell culture at concentrations of 10 μg/mL. The immunoliposomes incorporating the EME and docetaxel were active against the LNCap cells when exposed to heat at 39-40 °C. The liposomes not exposed to heat were inactive against the LNCap cells. The developed heat-sensitive immunoliposomes used for the delivery of both the EME and chemotherapeutic agents was able to successfully release the entrapped contents upon heat exposure above the phase transition temperature of the liposome membrane. The heat-sensitive immunoliposomes conjugated with a PSA antibody encapsulated the extract successfully and showed better cell antiproliferation efficacy against the prostate cancer cell lines in the presence of heat.

摘要

利用纳米技术递送抗癌药物是一种很有前景的方法，旨在提高治疗效果并降低化疗药物的毒性。使用HSPC:DSPE-PEG-2000:DSPE-PEG2000-马来酰亚胺以4:1:0.2的比例制备脂质体，并与前列腺特异性抗原（PSA）抗体偶联。研究了包封在温度敏感免疫脂质体中的艾地苯醌提取物（EME）、阿霉素（Dox）和多西他赛（Doc）对前列腺癌LNCap和DU145细胞系的活性。对EME叶、根和茎的有机提取物针对这两种细胞系进行筛选，在浓度为10μg/mL时可抑制超过50%的细胞培养。当在39 - 40°C加热时，包载EME和多西他赛的免疫脂质体对LNCap细胞有活性。未加热的脂质体对LNCap细胞无活性。所开发的用于递送EME和化疗药物的热敏免疫脂质体在高于脂质体膜相变温度的热暴露下能够成功释放包封的内容物。与PSA抗体偶联的热敏免疫脂质体成功包封了提取物，并在加热条件下对前列腺癌细胞系显示出更好的细胞抗增殖效果。

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