Liu Laipan, Shen Wenjing, Ren Zhentao, Fang Zhixiang, Zhang Li, Yin Xin, Yu Qi, Liu Biao
Key Laboratory on Biodiversity and Biosafety, Nanjing Institute of Environmental Sciences, MEE, Nanjing 210042, China.
Insects. 2024 Nov 4;15(11):861. doi: 10.3390/insects15110861.
Transgenic poplars have been used to control quarantine pests worldwide, such as the fall webworm (, FW). However, the studies on the resistance mechanism of FW to Cry toxins are limited. This study obtained an FW strain with 45-fold resistance to Cry1Ab toxin by continuous screening in the laboratory. The resistance to Cry1Ab was autosomally completely recessive, and it had approximately 40-fold cross-resistance to Cry1Ac but no cross-resistance to Cry2Ab toxin. Analysis of indoor population life tables for the resistant and susceptible strains found no significant difference in the net reproduction rate (R) between the two strains. The resistant strain had an insignificantly longer larval development duration and a comparable pupation rate, pupal weight, emergence rate, eggs laidper female, and egg hatching rate compared to the susceptible strain. This indicated that there was no fitness cost for Cry1Ab resistance. Our study helps in evaluating the risk of resistance to Cry1Ab toxin and the mechanisms of resistance to Cry1Ab toxin in FW.
转基因杨树已被用于全球范围内控制检疫性害虫，如美国白蛾（Hyphantria cunea，FW）。然而，关于美国白蛾对Cry毒素抗性机制的研究有限。本研究通过在实验室中连续筛选，获得了对Cry1Ab毒素具有45倍抗性的美国白蛾品系。对Cry1Ab的抗性为常染色体完全隐性，对Cry1Ac具有约40倍的交叉抗性，但对Cry2Ab毒素无交叉抗性。对抗性和敏感品系的室内种群生命表分析发现，两个品系的净繁殖率（R）无显著差异。与敏感品系相比，抗性品系的幼虫发育历期略长，化蛹率、蛹重、羽化率、单雌产卵量和卵孵化率相当。这表明Cry1Ab抗性不存在适合度代价。我们的研究有助于评估美国白蛾对Cry1Ab毒素的抗性风险以及对Cry1Ab毒素的抗性机制。
