Rouse G W, Robson S K
J Submicrosc Cytol. 1986 Jan;18(1):137-52.
The fine structure of epididymal or electro-ejaculated spermatozoa of Pteropus poliocephalus, P. scapulatus, P. conspiculatus, P. alecto and Syconycteris australis is described. The sperm of all species were found to be very similar. The head is extremely flattened and spatulate; the nucleus is capped by a long acrosome that comprises the proximal half of the head and covers two-thirds of the nucleus area, and a prominent sub-acrosomal space possesses a unique 'anvil'-like shape. Redundant nuclear envelope forms a 'scroll' in a restricted region of the neck, next to the base of the proximal centriole and the most proximal of the mitochondria. The axoneme is atypical for mammals, the central singlets arising distal to the outer doublets. Coarse fibres 1, 5, 6 and 9 are larger than the remainder, a feature shared with the Microchiroptera, most insectivores and the Primates. The large sub-acrosomal space of the megachiropteran sperm is significantly is different from that of the Microchiroptera. We consider that the difference in sperm ultrastructure between the chiropteran sub-orders is not inconsistent with theories of a di-phyletic origin for this group. The use of sperm ultrastructure as a phylogenetic tool is discussed and comparisons with the spermatozoa of other closely related Eutheria are made.
本文描述了灰头狐蝠、肩章狐蝠、显著狐蝠、黑狐蝠和澳洲管鼻果蝠附睾精子或电刺激采得精子的精细结构。发现所有物种的精子都非常相似。头部极度扁平且呈铲状；细胞核由一个长长的顶体覆盖，顶体占据头部的近端一半并覆盖细胞核面积的三分之二，一个突出的顶体下空间呈独特的“砧”状。多余的核膜在颈部的一个受限区域形成一个“卷轴”，紧邻近端中心粒基部和最靠近的线粒体。轴丝在哺乳动物中不典型，中央单微管起源于外周双微管的远端。粗纤维1、5、6和9比其余的更大，这一特征与小蝙蝠亚目、大多数食虫动物和灵长目动物相同。大蝙蝠亚目精子的大顶体下空间与小蝙蝠亚目显著不同。我们认为蝙蝠亚目的精子超微结构差异与该类群双系起源的理论并不矛盾。本文讨论了将精子超微结构用作系统发育工具的用途，并与其他亲缘关系较近的真兽亚纲动物的精子进行了比较。
