Zhang Zixuan, Zhang Zhuo, Zeng Wen, Li Yang, Zhu Chenhui
Shaanxi Key Laboratory of Degradable Biomedical Materials and Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China.
Plastic and Cosmetic Maxillofacial Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710065, China.
Int J Biol Macromol. 2024 Dec;283(Pt 4):138039. doi: 10.1016/j.ijbiomac.2024.138039. Epub 2024 Nov 26.
Melanoma treatment remains a challenge due to the inadequacy of existing clinical approaches and the difficulty of tissue regeneration. Recently, microneedles have been widely studied in tumor therapy and skin repair. Hence, a hyaluronic acid (HA)-based dual-functional hydrogel microneedle (MN) system was constructed to sequentially achieve tumor ablation and skin regeneration. Carbon nanotubes@calcium peroxide@tannic acid-Fe/chlorin e6 (CCa@TF/Ce6) nanomaterial was encapsulated in dissolvable polyvinyl alcohol/polyvinylpyrrolidone tips and accurately released to the tumor site to suppress melanoma via the photothermal and photodynamic therapies under the dual laser irradiation due to the generation of reactive oxygen species (ROS) and heat. Particularly, ROS and heat triggered immunogenic cell death, promoted dendritic cells maturation and reshaped the tumor-associated macrophage phenotype from the protumor M2 phenotype to the anti-tumor M1 phenotype, ultimately activating autoimmunity to eliminate the tumor. Following tumor ablation, the MN base accelerated skin regeneration owing to HB-PVA hydrogel through reducing oxidative stress, promoting cell proliferation and facilitating cell migration. Overall, the dual-functional hydrogel MN designed in this study, offers a new avenue for sequential melanoma combination treatment and skin regeneration.
由于现有临床方法的不足以及组织再生的困难，黑色素瘤治疗仍然是一项挑战。近年来，微针在肿瘤治疗和皮肤修复方面得到了广泛研究。因此，构建了一种基于透明质酸（HA）的双功能水凝胶微针（MN）系统，以依次实现肿瘤消融和皮肤再生。将碳纳米管@过氧化钙@单宁酸-Fe/氯e6（CCa@TF/Ce6）纳米材料封装在可溶解的聚乙烯醇/聚乙烯吡咯烷酮尖端，并在双激光照射下通过光热和光动力疗法准确释放到肿瘤部位，以抑制黑色素瘤，这是由于活性氧（ROS）和热量的产生。特别地，ROS和热量引发免疫原性细胞死亡，促进树突状细胞成熟，并将肿瘤相关巨噬细胞表型从促肿瘤M2表型重塑为抗肿瘤M1表型，最终激活自身免疫以消除肿瘤。肿瘤消融后，MN基部通过HB-PVA水凝胶加速皮肤再生，这是通过减少氧化应激、促进细胞增殖和促进细胞迁移实现的。总体而言，本研究设计的双功能水凝胶MN为黑色素瘤序贯联合治疗和皮肤再生提供了一条新途径。
