Hu Cong, Liu Hongwen, Zhang Zhipengjun, Li Lingyun, Mao Guo-Jiang, Cheng Wei, Zhou Lei
Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, P. R. China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, P. R. China.
Small. 2025 May;21(20):e2408527. doi: 10.1002/smll.202408527. Epub 2024 Nov 26.
Pancreatic cancer is known for its high invasiveness and metastasis, making rapid visualization and precise treatment critical for improving patient outcomes. Current diagnostic tools lack abilities to provide rapid and accurate tumor localization, particularly for real-time intraoperative guidance. To address this gap, the study has developed a novel Förster Resonance Energy Transfer (FRET)-mediated dual-ratiometric near-infrared fluorescence (NIRF)/photoacoustic (PA) bimodal probe, SiRho-SHD-NTR, specifically designed for the fast and accurate navigation of pancreatic tumor resection. The probe, due to its excellent binding affinity with nitroreductase (NTR), can rapidly reach response saturation. Cellular experiments demonstrate that the probe rapidly and efficiently penetrates cancer cells, enhancing the effectiveness of PA imaging for preliminary diagnosis and tumor localization, while also enabling the rapid visualization of pancreatic tumors through NIRF imaging. Leveraging the rapid response characteristics of the probe to NTR, the study achieves precise tumor imaging in orthotopic pancreatic cancer mice by spraying the probe, within ≈5 min. More importantly, the probe even allows for the fast visualization of metastatic tumors and fluorescence-guided surgical resection. It is believed that SiRho-SHD-NTR will offer a promising method in the rapid visualization of pancreatic cancer and provide a powerful tool for imaging-guided tumor surgery, targeting both primary and metastatic tumors.
胰腺癌以其高侵袭性和转移性而闻名，因此快速可视化和精确治疗对于改善患者预后至关重要。目前的诊断工具缺乏快速准确地进行肿瘤定位的能力，尤其是在实时术中引导方面。为了填补这一空白，该研究开发了一种新型的Förster共振能量转移（FRET）介导的双比率近红外荧光（NIRF）/光声（PA）双峰探针SiRho-SHD-NTR，专门用于胰腺肿瘤切除的快速准确导航。该探针由于与硝基还原酶（NTR）具有出色的结合亲和力，能够迅速达到反应饱和。细胞实验表明，该探针能快速有效地穿透癌细胞，增强PA成像用于初步诊断和肿瘤定位的效果，同时还能通过NIRF成像实现胰腺肿瘤的快速可视化。利用该探针对NTR的快速响应特性，该研究通过在约5分钟内喷洒探针，在原位胰腺癌小鼠中实现了精确的肿瘤成像。更重要的是，该探针甚至能够实现转移瘤的快速可视化以及荧光引导下的手术切除。相信SiRho-SHD-NTR将为胰腺癌的快速可视化提供一种有前景的方法，并为成像引导的肿瘤手术提供强大工具，可同时针对原发性和转移性肿瘤。
