Construction of biopolymer-based hydrogel beads for encapsulation, retention, and colonic delivery of tributyrin: Development of functional beverages (fortified bubble tea).

Tributyrin (TB) can be hydrolyzed into short chain fatty acids (butyric acid) in the gastrointestinal tract, which are claimed to exhibit beneficial health effects in the colon. However, digestion of tributyrin in the stomach and small intestine may promote its absorption in the upper gastrointestinal tract, thereby reducing its potential colonic health benefits. In this study, we therefore developed a novel method of encapsulating emulsified tributyrin within biopolymer-based hydrogel beads (≈ 800 μm) that were then encapsulated inside the boba beads (≈ 8-10 mm) found in bubble tea. The hydrogel beads were designed to retain and protect the tributyrin under upper gastrointestinal tract (GIT) conditions, but then release it within the colon. The concentration of tributyrin within the boba beads was 33.3 mg/g, which is above the value reported to exhibit health benefits. The morphology, encapsulation properties, water holding capacity, stability, and swelling properties of the tributyrin-loaded boba beads were characterized. Tapioca-based beads exhibited a larger degree of swelling when incubated in water for 12h (>95 %), whereas agar-based beads did not (< 20 %). In addition, the potential gastrointestinal fate of both free and encapsulated tributyrin oil droplets was assessed using an in vitro digestion model. The free tributyrin oil droplets were almost completely hydrolyzed (103.2 %) by the end of the small intestine phase, whereas the tributyrin oil droplets encapsulated within the agar-based (29.4 %) or tapioca-based (40.3 %) boba beads were much more resistant to digestion. The tapioca-based beads were partially broken down as they passed through the simulated GIT, while the agar-based beads maintained their structural integrity. The tapioca-based beads were gradually broken down as they passed through the simulated GIT, while the agar-based ones maintained their structural integrity. Agar beads were also harder, more resilient, and chewier than the tapioca ones. Both types of boba beads tended to swell and disintegrate when heated to high temperatures (90 °C), with the effect being more pronounced for the agar beads. Overall, our results suggest that the agar-based boba beads had greater potential for the delivery of tributyrin to the colon than the tapioca-based ones. The recent popularity of bubble tea means that it may be a suitable vehicle for delivering bioactive food components, like functional lipids, vitamins, nutraceuticals, or probiotics.