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预酸化诱导对即食鹅肉理化特性、肌原纤维蛋白微观结构和顶空挥发性的影响。

Effect of pre-acidification induction on the physicochemical features, myofibrillar protein microstructure, and headspace volatiles of ready-to-cook goose meat.

机构信息

Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, College of Food Science and Technology, Ningbo University, Ningbo 315211, China.

State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, China.

出版信息

Food Res Int. 2024 Dec;197(Pt 1):115166. doi: 10.1016/j.foodres.2024.115166. Epub 2024 Oct 5.

Abstract

This study examined the impact of pre-acidification induction on the quality attributes and flavor retention of ready-to-cook (RTC) goose meat products. The results demonstrated that pre-acidification could influence the eating qualities of RTC goose meat by effectively regulating the physicochemical properties of goose myofibrillar proteins (MP) including solubility and water-holding capacity. Elevated carbonyl contents indicated an enhanced gel-forming capacity in RTC goose meat during storage, accompanied with reduced total sulfhydryl contents from enhanced protonation pretreatment and augmented lipid oxidation. Structural characterization of MP via sodium dodecyl sulfate-polyacrylamide gel electrophoresis, circular dichroism spectroscopy, and intrinsic fluorescence revealed the formation of a dense protein matrix under highly acidic conditions. Furthermore, the headspace concentration of aldehydes increased by 3.23 times upon enhancing the pre-acidification intensity, resulting in the production of esters and acidic flavor compounds with favorable aromas. Correlation analysis demonstrated the dependence of headspace concentrations of volatile constituents on the acidification-enhanced surface hydrophobicity of MP, attributed to the modified binding sites of proteins after pre-acidification. Current results have indicated both the positive and negative influence of pre-acidulation induction on the eating quality of goose meat products, suggesting the necessity of introducing extra processes to modulate the quality of prefabricated products.

摘要

本研究探讨了预酸化诱导对即食(RTC)鹅肉产品质量属性和风味保持的影响。结果表明,预酸化可以通过有效调节鹅肌原纤维蛋白(MP)的理化性质,包括溶解度和持水力,来影响 RTC 鹅肉的食用品质。羰基含量的升高表明 RTC 鹅肉在储存过程中的凝胶形成能力增强,同时由于质子化预处理增强和脂质氧化增加,总巯基含量降低。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、圆二色性光谱和内源荧光对 MP 的结构特征进行表征,发现 MP 在高度酸性条件下形成了致密的蛋白质基质。此外,通过增强预酸化强度,醛的顶空浓度增加了 3.23 倍,从而产生了具有良好香气的酯类和酸性风味化合物。相关分析表明,挥发性成分的顶空浓度取决于 MP 酸化增强的表面疏水性,这归因于预酸化后蛋白质结合位点的改变。目前的结果表明,预酸化诱导对鹅肉产品食用品质既有积极影响,也有消极影响,这表明需要引入额外的工艺来调节预制产品的质量。

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