Generation of a synthetic autochthonous microbiota responsible for the essential flavors and brewing properties of Daqu.

Based on the 26 strains of medium-high-temperature Daqu, seed microbial inocula and mature microbial inoculants were prepared and inoculated into crushed wheat to prepare cleaner medium-high-temperature Daqu (XQ). The physicochemical properties, flavor, and microbial profile of XQ were studied using traditional medium-high-temperature Daqu (CQ) or industry standards as controls. The results showed that the liquefaction ability, saccharification activity, esterification activity, and moisture content of XQ were better than those of Daqu industry standards. The results of GC-MS and E-nose analyses showed that the main flavor compounds in the two types of Daqu were esters, alcohols, acids, and aldehydes, which had similar flavor profiles. Microbial sequencing revealed that the most abundant fungi in XQ were Aspergillus subflavus, Wickerhamomyces anomalus, Rhizopus oryzae, and Candida tropicalis, whereas the most abundant bacteria were Lactobacillus curvatus, Weissella cibaria, and Bacillus subtilis. Both types of Daqu contained a high abundance of W. anomalus. In addition, the COG (Clusters of Orthologous Groups of Proteins) analyses displayed that the two types of Daqu had their dominant functions and generally had the pathways and functional characteristics of organism growth and metabolism. This study laid some experimental research bases for the preparation of cleaner Daqu and could provide insights for producing safer microbial fermented foods.