Wu Ying, Gao Dejiao, Pan Yujia, Dong Yao, Bai Zhouya, Gu Shaobin
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China.
Henan Engineering Research Center of Food Microbiology, Luoyang 471000, China.
Foods. 2024 Nov 17;13(22):3655. doi: 10.3390/foods13223655.
Obesity, a prevalent metabolic disorder in youth, leads to complications and economic strain. Gut dysbiosis significantly contributes to obesity and metabolic issues. , a probiotic strain, may help regulate gut dysbiosis and benefit obese individuals. However, more research is needed on its effect on serum metabolism. A total of 75 overweight or obese young adults (aged 19-45) participated in the current study, and were randomly divided into probiotic and placebo groups using a random number table. Both groups received dietary guidance and underwent twelve weeks of intervention with either oral BBr60 (BBr60) or a placebo. After the intervention, collection and analysis of fasting serum samples were conducted using mass spectrometry coupled with liquid chromatography. Analyses of associations were conducted in order to determine the correlations between key serum metabolites and clinical obesity indicators, aiming to understand the influence of BBr60. Due to 10 participants dropping out for personal reasons, the study included 32 and 33 participants in the placebo and the BBr60 groups, respectively. The BBr60 intervention significantly regulated 134 serum metabolites and influenced crucial metabolic pathways in obesity management ( < 0.05), including ascorbate and aldarate metabolism for oxidative stress reduction, cholesterol metabolism for lipid regulation, parathyroid hormone synthesis, secretion and action for the endocrine system, oxidative phosphorylation for enhanced energy efficiency, and glycolysis/gluconeogenesis for glucose metabolism. Analysis showed a positive relationship between fasting blood glucose (FBG), aspartate aminotransferase (AST), total protein (TP), and the content of 5-Methyl DL-glutamate ( < 0.05). Similarly, body mass index (BMI), weight, and body fat percentage (BFP) were positively linked to serum metabolites (1-Hydroxycyclohexyl) acetic acid, and 5-Oxooctanoic acid ( < 0.05). Significant associations of AST levels with key serum metabolites in cholesterol metabolism pathways further suggest BBr60's potential to improve liver function and overall metabolic health in overweight or obese individuals. These findings support BBr60's effectiveness in modulating serum metabolic profiles and suggest it may improve liver function and BMI in overweight or obese individuals by regulating key serum metabolites.
肥胖是青少年中一种普遍存在的代谢紊乱疾病，会导致并发症和经济负担。肠道菌群失调在很大程度上导致肥胖和代谢问题。BBr60作为一种益生菌菌株，可能有助于调节肠道菌群失调并使肥胖个体受益。然而，关于其对血清代谢影响的研究还需要更多。共有75名超重或肥胖的年轻人（年龄在19 - 45岁之间）参与了本研究，并使用随机数字表随机分为益生菌组和安慰剂组。两组均接受饮食指导，并分别口服BBr60或安慰剂进行了为期十二周的干预。干预后，使用液相色谱 - 质谱联用技术对空腹血清样本进行收集和分析。进行关联分析以确定关键血清代谢物与临床肥胖指标之间的相关性，旨在了解BBr60的影响。由于10名参与者因个人原因退出，该研究在安慰剂组和BBr60组中分别纳入了32名和33名参与者。BBr60干预显著调节了134种血清代谢物，并影响了肥胖管理中的关键代谢途径（P < 0.05），包括用于减轻氧化应激的抗坏血酸和醛糖代谢、用于脂质调节的胆固醇代谢、甲状旁腺激素的合成、分泌和作用以调节内分泌系统、用于提高能量效率的氧化磷酸化以及用于葡萄糖代谢的糖酵解/糖异生。分析显示空腹血糖（FBG）、天冬氨酸转氨酶（AST）、总蛋白（TP）与5 - 甲基DL - 谷氨酸含量之间存在正相关关系（P < 0.05）。同样，体重指数（BMI）、体重和体脂百分比（BFP）与血清代谢物（1 - 羟基环己基）乙酸和5 - 氧代辛酸呈正相关（P < 0.05）。AST水平与胆固醇代谢途径中的关键血清代谢物之间的显著关联进一步表明BBr60在改善超重或肥胖个体肝功能和整体代谢健康方面的潜力。这些发现支持了BBr60在调节血清代谢谱方面的有效性，并表明它可能通过调节关键血清代谢物来改善超重或肥胖个体的肝功能和BMI。
