Institute of Clinical Medicine, Clinical Pathology and Forensic Medicine, University of Eastern Finland, FI-70211 Kuopio, Finland.
Cancer Center, Department of Oncology, Kuopio University Hospital, FI-70029 Kuopio, Finland.
Cells. 2024 Nov 14;13(22):1884. doi: 10.3390/cells13221884.
Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with a high tendency for developing a recurrent disease. Circulating micro-RNAs (cmiRNAs) obtained through liquid biopsy are potential prognostic biomarkers for the assessment of TNBC recurrence risk. In this study, we sequenced cmiRNAs from the serum samples of 14 recurrent and 19 non-recurrent TNBC cases and compared expression profiles in relation to recurrence status, survival data and miRNA expression in matched tumor samples. Differential expression analysis between recurrent and non-recurrent cases identified ten differentially expressed (DE) cmiRNAs, of which cmiRNAs miR-21-5p ( = 0.030, HR = 1.87, 95% CI 1.06-3.30), miR-16-5p ( = 0.032, HR = 0.53, 95% CI 0.30-0.95), and miR-26b-5p ( = 0.023, HR = 0.52, 95% CI 0.29-0.91) were associated with recurrence-free survival in multivariable survival analysis. Expression profiles of matched tumor and serum samples were shown to correlate with each other. DE cmiRNAs were associated with common cancer-related signaling pathways and improved the overall predictive performance of the logistic regression model assessing the recurrence risk. Our results indicate that recurrent and non-recurrent TNBC differ in their cmiRNA expression profiles, and that three specific cmiRNAs can be used to assess the risk of recurrence in TNBC.
三阴性乳腺癌（TNBC）是一种侵袭性乳腺癌亚型，具有较高的复发倾向。通过液体活检获得的循环 microRNA（cmiRNA）是评估 TNBC 复发风险的潜在预后生物标志物。在这项研究中，我们对 14 例复发和 19 例非复发 TNBC 病例的血清样本进行了 cmiRNA 测序，并比较了与复发状态、生存数据和匹配肿瘤样本中 miRNA 表达相关的表达谱。复发和非复发病例之间的差异表达分析确定了十个差异表达（DE）的 cmiRNA，其中 cmiRNA miR-21-5p（ = 0.030，HR = 1.87，95%CI 1.06-3.30）、miR-16-5p（ = 0.032，HR = 0.53，95%CI 0.30-0.95）和 miR-26b-5p（ = 0.023，HR = 0.52，95%CI 0.29-0.91）与多变量生存分析中的无复发生存相关。匹配的肿瘤和血清样本的表达谱显示彼此相关。DE cmiRNA 与常见的癌症相关信号通路相关，并提高了评估复发风险的逻辑回归模型的整体预测性能。我们的研究结果表明，复发和非复发的 TNBC 在其 cmiRNA 表达谱上存在差异，并且三种特定的 cmiRNA 可用于评估 TNBC 的复发风险。
