Institute of Biotechnology, Xinjiang Academy of Animal Science, Xinjiang Key Laboratory of Animal Biotechnology, Urumqi 830026, China.
College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China.
Int J Mol Sci. 2024 Nov 8;25(22):11988. doi: 10.3390/ijms252211988.
Skeletal muscle development is crucial for livestock production, and understanding the molecular mechanisms involved is essential for enhancing muscle growth in sheep. This study aimed to investigate the role of , a member of the nuclear factor I (NFI) family, in regulating muscle development in sheep, filling a significant gap in the current understanding of deficiency and its impact on skeletal muscle growth, as no similar studies have been reported in this species. Bioinformatic analysis, including temporal analysis of transcriptome data, identified as a potential target gene for muscle growth regulation. The effects of overexpression and knockout on the proliferation and differentiation of sheep skeletal muscle cells were investigated. Changes in the expression of associated marker genes were assessed to explore the regulatory link between and the myostatin () gene. Additionally, target miRNAs for and were predicted using online databases such as miRWalk, resulting in the construction of an -miRNA- interactive regulatory network. The findings revealed that promotes the proliferation and differentiation of sheep skeletal muscle cells, with further analysis indicating that may regulate muscle cell development by modulating expression. This study provides preliminary insights into the function of in sheep skeletal muscle development and its regulatory interactions, addressing a critical knowledge gap regarding deficiency and its implications for muscle growth. These findings contribute to a better understanding of muscle biology in sheep and provide a theoretical foundation for future research into the regulatory mechanisms governing muscle development.
骨骼肌发育对于畜牧业生产至关重要，深入了解相关的分子机制对于提高绵羊肌肉生长至关重要。本研究旨在探究核因子 I (NFI)家族成员 在调控绵羊肌肉发育中的作用，填补了当前对 缺乏及其对骨骼肌生长影响认识的空白，因为在该物种中尚未报道过类似的研究。通过生物信息学分析，包括转录组数据的时间分析，鉴定出 是肌肉生长调控的潜在靶基因。研究了 过表达和敲除对绵羊骨骼肌细胞增殖和分化的影响。评估相关标记基因的表达变化，以探讨 与肌肉生长抑制素 ( )基因之间的调控关系。此外，使用 miRWalk 等在线数据库预测了 和 的靶 miRNAs，构建了 -miRNA 相互作用调控网络。研究结果表明， 促进绵羊骨骼肌细胞的增殖和分化，进一步分析表明 可能通过调节 的表达来调控肌肉细胞的发育。本研究初步揭示了 在绵羊骨骼肌发育中的功能及其调控相互作用，填补了 缺乏及其对肌肉生长影响的关键知识空白。这些发现有助于深入了解绵羊肌肉生物学，并为研究调控肌肉发育的机制提供理论基础。
