Department of Science of Dental Materials, Dow Dental College, Dow University of Health Sciences, Karachi 74200, Pakistan.
Science of Dental Materials, Dr. Ishrat ul Ebad Khan Institute of Oral Health Sciences, Dow University of Health Sciences, Karachi 74200, Pakistan.
Int J Mol Sci. 2024 Nov 8;25(22):11991. doi: 10.3390/ijms252211991.
Intracanal medicaments are an important adjunct to the effective disinfection of the root canal system. However, conventional intracanal medicaments do not provide adequate protection against , which is the organism of interest in many cases of root canal failures. This study aimed to evaluate the influence of biosynthesized calcium oxide nanoparticles (CaO NPs) on the antibacterial activity, pH, microleakage and cytotoxicity of intracanal medicaments. CaO NPs were biosynthesized by the direct thermal decomposition of eggshells (EGS) and the reduction of calcium nitrate with papaya leaf extract (PLE). These nanoparticles were mixed with a proprietary calcium hydroxide powder in 10% and 25% (/) concentrations and blended in analytical-grade coconut oil to formulate the experimental medicaments. These were then evaluated for antibacterial activity, pH, microleakage and cytotoxicity at 1 day, 7 days and 15 days. A proprietary calcium hydroxide paste formulation (MX) was used as the control. Means and standard deviations were calculated and analyzed using repeated-measures ANOVA for pH and three-way ANOVA for the antibacterial effect, microleakage and cytotoxicity, followed by LSD post hoc analysis. Significant antibacterial activity was noted against at all times, with zones of inhibition (ZOI) up to 19.60 ± 2.30 mm. pH levels up to 13.13 ± 0.35 were observed for the experimental groups. Microleakage remained comparable to the control, while cytotoxicity was not observed in any of the groups at any time. Intracanal medicaments formulated with 10% and 25% (/) of biosynthesized CaO NPs could be promising candidates for the disinfection of the root canal system compared to conventional counterparts.
根管内药物是有效消毒根管系统的重要辅助手段。然而，传统的根管内药物并不能提供足够的保护，而在许多根管失败病例中，是感兴趣的生物体。本研究旨在评估生物合成氧化钙纳米粒子（CaO NPs）对根管内药物的抗菌活性、pH 值、微渗漏和细胞毒性的影响。CaO NPs 通过蛋壳（EGS）的直接热分解和番木瓜叶提取物（PLE）还原硝酸钙合成。将这些纳米颗粒以 10%和 25%（/）的浓度与专有氢氧化钙粉末混合，并在分析级椰子油中混合，以配制实验性药物。然后在第 1 天、第 7 天和第 15 天评估它们的抗菌活性、pH 值、微渗漏和细胞毒性。专有氢氧化钙糊剂配方（MX）用作对照。使用重复测量 ANOVA 计算和分析 pH 值，使用三因素 ANOVA 分析抗菌效果、微渗漏和细胞毒性，然后进行 LSD 事后分析。所有时间点均对 显示出显著的抗菌活性，抑菌圈（ZOI）高达 19.60±2.30mm。实验组的 pH 值高达 13.13±0.35。微渗漏与对照组相当，而在任何时间点都未观察到任何组的细胞毒性。与传统根管内药物相比，用 10%和 25%（/）生物合成 CaO NPs 配制的根管内药物可能是根管系统消毒的有前途的候选药物。
