School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
Int J Mol Sci. 2024 Nov 14;25(22):12213. doi: 10.3390/ijms252212213.
The risk of contamination is expanding with global warming. Targeting the pathogenicity of at its source and diminishing its colonization within the host may be a potential control strategy. Oxidative stress transcription factor AtfA plays a pivotal role in pathogenicity by combating reactive oxygen species (ROS) generated by host immune cells. This study employed chromatin immunoprecipitation sequencing to elucidate the binding sites and epigenetic mechanisms of AtfA under oxidative stress. Among the total 1022 identified potential AtfA-binding peaks, a 10-bp region predominated by 5'-DRTGTTGCAA-3', which is highly similar to the AP-1 binding motif was predicted. The significantly regulated genes exhibited a variety of biological functions, including regulation of filamentous growth, response to extracellular stimulus, and regulation of gene expression. Moreover, AtfA indirectly influenced these processes via the MAPK signaling pathway, carbon metabolism, and fatty acid metabolism in response to oxidative stress. The absence of contributed to the decrease in the growth and development, sporulation, AFB biosynthesis, and invasion ability of under oxidative stress. These findings suggest that AtfA is critical to overcome oxidative stress induced by the host immune cells during the infection, providing a novel target for early prevention of contamination.
污染风险随着全球变暖而扩大。针对病原体的致病性并减少其在宿主中的定植可能是一种潜在的控制策略。氧化应激转录因子 AtfA 通过与宿主免疫细胞产生的活性氧（ROS）作斗争，在致病性方面发挥着关键作用。本研究采用染色质免疫沉淀测序来阐明 AtfA 在氧化应激下的结合位点和表观遗传机制。在总共鉴定出的 1022 个潜在的 AtfA 结合峰中，一个由 5'-DRTGTTGCAA-3'组成的 10 个碱基区域占主导地位，这与 AP-1 结合基序高度相似。显著调节的基因表现出多种生物学功能，包括丝状生长的调节、对外界刺激的反应以及基因表达的调节。此外，AtfA 通过 MAPK 信号通路、碳代谢和脂肪酸代谢对这些过程产生间接影响，以响应氧化应激。缺乏 导致 在氧化应激下的生长发育、孢子形成、AFB 生物合成和侵袭能力下降。这些发现表明，AtfA 对于克服宿主免疫细胞在感染过程中诱导的氧化应激至关重要，为早期预防污染提供了一个新的靶点。
