R&BD Center, Hy Co., Ltd., 22 Giheungdanji-ro 24 Beon-gil, Giheung-gu, Yongin-si 17086, Republic of Korea.
Int J Mol Sci. 2024 Nov 15;25(22):12282. doi: 10.3390/ijms252212282.
Dry eye is characterized by persistent instability and decreased tear production, which are accompanied by epithelial lesions and inflammation on the surface of the eye. In our previous paper, we reported that supplementation with HY7302 (HY7302) could inhibit corneal damage in a benzalkonium chloride (BAC)-induced mouse model of dry eye, through its effects in gut microbiome regulation. The aim of this study was to determine what functional extracellular substances can alter the inflammatory response of conjunctival cells. We isolated exosomes from HY7302 probiotic culture supernatant, analyzed their morphological characteristics, and found that their average size was 143.8 ± 1.1 nm, which was smaller than the exosomes from the KCTC 3112 strain. In addition, HY7302-derived exosomes significantly reduced the levels of genes encoding pro-inflammatory cytokines, including , , , and , in BAC-treated human conjunctival cells. Moreover, HY7302-derived exosomes significantly increased the levels of genes encoding tight junction proteins, including , , and , in Caco-2 cells. Lastly, the HY7302 exosomes reduced mRNA expression levels of , , , , and in a transwell coculture system. Our findings indicate that HY7302 exosomes have potential for use in the treatment of ocular inflammation-related dry eye disease, through gut-eye axis communication via exosomes.
干眼症的特征为持续性不稳定和泪液产生减少，同时伴有眼表面上皮损伤和炎症。在我们之前的论文中，我们报道了 HY7302（HY7302）补充剂可以通过调节肠道微生物组来抑制苯扎氯铵（BAC）诱导的干眼症小鼠模型中的角膜损伤。本研究旨在确定哪些功能细胞外物质可以改变结膜细胞的炎症反应。我们从 HY7302 益生菌培养上清液中分离出外泌体，分析其形态特征，发现其平均大小为 143.8±1.1nm，小于 KCTC 3112 株的外泌体。此外，HY7302 衍生的外泌体显著降低了 BAC 处理的人结膜细胞中编码促炎细胞因子的基因的水平，包括 、 、 和 。此外，HY7302 衍生的外泌体显著增加了 Caco-2 细胞中编码紧密连接蛋白的基因的水平，包括 、 、和 。最后，HY7302 外泌体在 Transwell 共培养系统中降低了 、 、 、 和 的 mRNA 表达水平。我们的研究结果表明，HY7302 外泌体通过外泌体通过肠道-眼睛轴通讯，在治疗与眼部炎症相关的干眼症方面具有潜力。
