Panis Nele, Gestels Zina, Van Den Bossche Dorien, De Baetselier Irith, Abdellati Said, Vanbaelen Thibaut, de Block Tessa, Santhini Manoharan-Basil Sheeba, Kenyon Chris
STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium.
Clinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium.
Antibiotics (Basel). 2024 Nov 18;13(11):1097. doi: 10.3390/antibiotics13111097.
: The use of antimicrobials to treat food animals leaves antimicrobial residues in foodstuffs. The World Health Organization (WHO) defines the acceptable daily intakes (ADIs) of these residues as the dose of these antimicrobials that is safe for an average human to consume on a daily basis. We hypothesized that the lowest dose of ciprofloxacin classified as safe by the WHO could select for ciprofloxacin-resistant strains of in a model. : We aimed to evaluate if the consumption of peri-ADI doses of ciprofloxacin could select for ciprofloxacin-resistant (Ser464Phe, GyrB, ciprofloxacin MIC of 4 µg/mL) compared to -susceptible (isogenic, ciprofloxacin MIC of 0.047 µg/mL) strains of in a model. : A significant increase was seen in the proportion of resistance for the 1× ADI and 1/10th ADI concentrations on day 2 compared to the positive control. : A model of infection in larvae was used for the experiment. The larvae were inoculated with followed by 10× ADI, 1× ADI, 1/10th ADI, 1/100th ADI, and 1/1000th ADI doses of ciprofloxacin. The isolation of colonies was then performed on selective agar plates with and without ciprofloxacin (1 µg/mL). The proportion of colonies with ciprofloxacin resistance was then calculated for each group at 24 and 48 h. : We found that, at 48 h, there was an enrichment of colonies with ciprofloxacin resistance in the larvae receiving 1× ADI and 1/10th ADI concentrations of ciprofloxacin. These results suggest that the ciprofloxacin MSC for in this model is 1/10th of the acceptable daily concentration (ADI) dose of ciprofloxacin, which is equivalent to 0.239 ng/µL.
使用抗菌药物治疗食用动物会在食品中留下抗菌药物残留。世界卫生组织（WHO）将这些残留的每日可接受摄入量（ADI）定义为普通人每天食用安全的这些抗菌药物剂量。我们假设，WHO分类为安全的环丙沙星最低剂量可在一个模型中选择出对环丙沙星耐药的菌株。：我们旨在评估与模型中对环丙沙星敏感（同基因，环丙沙星MIC为0.047μg/mL）的菌株相比，食用接近ADI剂量的环丙沙星是否能选择出对环丙沙星耐药（Ser464Phe，GyrB，环丙沙星MIC为4μg/mL）的菌株。：与阳性对照相比，在第2天，1×ADI和1/10 ADI浓度下的耐药比例显著增加。：实验使用了家蝇幼虫感染模型。给幼虫接种后，分别给予10×ADI、1×ADI、1/10 ADI、1/100 ADI和1/1000 ADI剂量的环丙沙星。然后在含和不含环丙沙星（1μg/mL）的选择性琼脂平板上进行家蝇菌落分离。然后计算每组在24小时和48小时时具有环丙沙星耐药性的菌落比例。：我们发现，在48小时时，接受1×ADI和1/10 ADI浓度环丙沙星的幼虫中，具有环丙沙星耐药性的家蝇菌落有所富集。这些结果表明，在该模型中家蝇对环丙沙星的最低选择浓度（MSC）是环丙沙星每日可接受浓度（ADI）剂量的1/10，相当于0.239 ng/μL。
