Luna-Rojas Sofía L, Vázquez-Guerrero Edwin, Díaz-Aparicio Efrén, Cantú-Covarrubias Antonio, Hernández-Castro Rigoberto, Álvarez-Hernández Gerardo, Estrada-de Los Santos Paulina, Ibarra J Antonio
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México.
Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Ciudad de México, México.
Vector Borne Zoonotic Dis. 2025 Mar;25(3):180-186. doi: 10.1089/vbz.2024.0098. Epub 2024 Nov 26.
spp. are obligate intracellular bacteria of utmost importance for public health and the economy since their presence can generate significant losses in livestock production, affecting the health of animals, the quality of the meat, milk, and other products derived from affected animals. Therefore, prevention of the transmission of these pathogens, their early identification and timely treatment are essential to mitigate their impact on public health and animal production. In the present work, hard ticks were collected from infested cattle in Aldama, Tamaulipas, Mexico. They were identified by morphology using dichotomous keys and by sequencing and analyzing of a fragment of the mitochondrial 16S. PCR was performed using specific primers targeting sp. . Phylogenetic analyses were performed, aligning the amino acid sequences with Muscle, and a phylogenetic tree was generated using PhyML. and ticks infesting the cattle were identified. Using molecular techniques, the presence of was identified in the cohort of analyzed ticks, suggesting a circulation of this pathogen in livestock in this region and granting further research in this area.
某些物种是专性细胞内细菌,对公共卫生和经济至关重要,因为它们的存在会给畜牧业生产造成重大损失,影响动物健康、肉类、牛奶及其他源自受感染动物的产品质量。因此,预防这些病原体的传播、早期识别和及时治疗对于减轻它们对公共卫生和动物生产的影响至关重要。在本研究中,硬蜱采自墨西哥塔毛利帕斯州阿尔达马受感染的牛。通过使用二歧检索表进行形态学鉴定,并对线粒体16S的一个片段进行测序和分析来鉴定它们。使用针对某些物种的特异性引物进行PCR。进行了系统发育分析,用Muscle比对氨基酸序列,并使用PhyML生成系统发育树。鉴定出了感染牛的某些蜱类。通过分子技术,在分析的蜱类群体中鉴定出了某些物种的存在,这表明该病原体在该地区的家畜中存在循环,值得在该领域进一步研究。