Rossi Vittorio, Taibi Othmane, Furiosi Margherita, Caffi Tito
Research Center for Plant Health Modelling (PHeM), Department of Sustainable Crop Production, 29100 Piacenza, Italy.
Università Cattolica del Sacro Cuore, 29100 Piacenza, Italy.
Plant Dis. 2025 Jun;109(6):1236-1243. doi: 10.1094/PDIS-07-24-1575-RE. Epub 2025 Jun 5.
Plant resistance inducers (PRIs) are promising alternatives to chemical fungicides. Their effectiveness against grapevine downy mildew (DM) has been demonstrated for leaves, yet research on berry clusters is limited. We investigated the efficacy of six PRIs on clusters of the cultivars Barbera and Merlot from the end of flowering (growth stage [GS] 69) to fruit setting (GS 71) and inoculated with a sporangial suspension of () at 1, 3, 6, 12, and 19 days after treatment (DAT). Cerevisane (CER) and (PYT) did not reduce DM severity compared with the nontreated control (NT). Fosetyl-Al (FOS) reduced DM severity at 1 to 6 DAT, with >50% reduction compared with NT. Laminarin (LAM) was effective at 6, 12, and 19 DAT, with 42.7 to 50.0% efficacy compared with NT, while cos-oga (COS) and K-phosphonate (PHO) were effective at 12 and 19 DAT (60% efficacy). PRIs were also applied to clusters at initial flowering (GS 60), GS 69 to 71, and pea-sized berries (GS 75), which were then inoculated with at 7 DAT. At GS 60, treatments with LAM and PHO reduced disease severity compared with NT by 77.5 and 83.6%, respectively. At GS 69 to 71, LAM, PHO, and FOS caused 54.7 to 75.7% disease reduction. At GS 75, all PRIs exhibited a disease reduction >75%. The efficacy of PRIs increased as the cluster developmental stage advanced, indicating an interaction with the ontogenic resistance of berries. Our results are relevant for the practical use of PRIs in protecting grapevine clusters from DM.
植物抗性诱导剂（PRIs）是化学杀菌剂很有前景的替代品。它们对葡萄霜霉病（DM）在叶片上的防治效果已得到证实，但对果穗的研究有限。我们研究了六种PRIs在开花末期（生长阶段[GS]69）至坐果期（GS 71）对巴贝拉和梅洛品种果穗的功效，并在处理后1、3、6、12和19天（DAT）用（）的孢子囊悬浮液进行接种。与未处理对照（NT）相比，Cerevisane（CER）和（PYT）并未降低霜霉病严重程度。福美双铝（FOS）在处理后1至6天降低了霜霉病严重程度，与NT相比降低了50%以上。海带多糖（LAM）在处理后6、12和19天有效，与NT相比防治效果为42.7%至50.0%，而cos-oga（COS）和钾膦酸盐（PHO）在处理后12和19天有效（防治效果60%）。PRIs还在初花期（GS 60）、GS 69至71以及豌豆大小的浆果期（GS 75）施用于果穗，然后在处理后7天用进行接种。在GS 60时，与NT相比，LAM和PHO处理分别使病害严重程度降低了77.5%和83.6%。在GS 69至71时，LAM, PHO和FOS使病害降低了54.7%至75.7%。在GS 75时，所有PRIs均表现出病害降低>75%。随着果穗发育阶段的推进，PRIs的功效增加，表明其与浆果的个体发育抗性存在相互作用。我们的结果对于PRIs在保护葡萄果穗免受霜霉病侵害方面的实际应用具有参考价值。
